Plant Transcription factor database - Universität Potsdam
version: 3.0

Populus trichocarpa GeBP Family

Description

Curaba et al. 2003: Trichomes of Arabidopsis are single-celled epidermal hair that are a useful model for studying plant cell fate determination. Trichome initiation requires the activity of the GLABROUS1 (GL1) gene whose expression in epidermal and trichome cells is dependent on the presence of a 3'-cis-regulatory element. Using a one-hybrid screen, we have isolated a cDNA, which encodes for a protein, GL1 enhancer binding protein (GeBP), that binds this regulatory element in yeast and in vitro. GeBP and its three homologues in Arabidopsis share two regions: a central region with no known motifs and a C-terminal region with a putative leucine-zipper motif. We show that both regions are necessary for trans-activation in yeast. A translational fusion with the Yellow Fluorescent Protein (YFP) indicates that GeBP is a nuclear protein whose localization is restricted to, on average, 3-5 subnuclear foci that might correspond to nucleoli. Transcriptional fusion with the GUS reporter indicates that GeBP is mainly expressed in vegetative meristematic tissues and in very young leaf primordia. We looked at GeBP expression in plants mutated in or misexpressing KNAT1, a KNOX gene, expressed in the shoot apical meristem and downregulated in leaf founder cells, and found that GeBP transcript level is regulated by KNAT1 suggesting that KNAT1 is a transcriptional activator of GeBP. This regulation suggests that GeBP is acting as a repressor of leaf cell fate.

Members of this family
  SHOULD possess DUF573 domain

Domain alignments

This family is also present in:

References

There are 7 gene models in this family



Gene modelDescriptionDomains
557514 eugene3.00090774 DUF573
558529 eugene3.00050144 DUF573
650024 grail3.0001096701 DUF573
752281 fgenesh4_pg.C_LG_I001884 DUF573
753066 fgenesh4_pg.C_LG_I002669 DUF573
759027 fgenesh4_pg.C_LG_IV000655 DUF573
763915 fgenesh4_pg.C_LG_VII000260 DUF573

General references

Chevalier, F; Perazza, D; Laporte, F; Le Hénanff, G; Hornitschek, P; Bonneville, JM; Herzog, M; Vachon, G. 2008. GeBP and GeBP-like proteins are noncanonical leucine-zipper transcription factors that regulate cytokinin response in Arabidopsis. Plant Physiol. 146(3):1142-54 PUBMEDID:18162594
Curaba, J; Herzog, M; Vachon, G. 2003. GeBP, the first member of a new gene family in Arabidopsis, encodes a nuclear protein with DNA-binding activity and is regulated by KNAT1. Plant J. 33(2):305-17 PUBMEDID:12535344