Plant Transcription factor database - Universität Potsdam
version: 3.0

Zea mays GeBP Family

Description

Curaba et al. 2003: Trichomes of Arabidopsis are single-celled epidermal hair that are a useful model for studying plant cell fate determination. Trichome initiation requires the activity of the GLABROUS1 (GL1) gene whose expression in epidermal and trichome cells is dependent on the presence of a 3'-cis-regulatory element. Using a one-hybrid screen, we have isolated a cDNA, which encodes for a protein, GL1 enhancer binding protein (GeBP), that binds this regulatory element in yeast and in vitro. GeBP and its three homologues in Arabidopsis share two regions: a central region with no known motifs and a C-terminal region with a putative leucine-zipper motif. We show that both regions are necessary for trans-activation in yeast. A translational fusion with the Yellow Fluorescent Protein (YFP) indicates that GeBP is a nuclear protein whose localization is restricted to, on average, 3-5 subnuclear foci that might correspond to nucleoli. Transcriptional fusion with the GUS reporter indicates that GeBP is mainly expressed in vegetative meristematic tissues and in very young leaf primordia. We looked at GeBP expression in plants mutated in or misexpressing KNAT1, a KNOX gene, expressed in the shoot apical meristem and downregulated in leaf founder cells, and found that GeBP transcript level is regulated by KNAT1 suggesting that KNAT1 is a transcriptional activator of GeBP. This regulation suggests that GeBP is acting as a repressor of leaf cell fate.

Members of this family
  SHOULD possess DUF573 domain

Domain alignments

This family is also present in:

References

There are 15 gene models in this family



Gene modelDescriptionDomains
GRMZM2G020408_P01 DUF573
GRMZM2G026417_P01 DUF573
GRMZM2G029965_P01 DUF573
GRMZM2G030458_P01 DUF573
GRMZM2G041818_P01 DUF573
GRMZM2G049899_P01 DUF573
GRMZM2G075122_P01 DUF573
GRMZM2G082318_P01 DUF573
GRMZM2G092409_P01 DUF573
GRMZM2G121466_P01 DUF573
GRMZM2G134866_P01 DUF573
GRMZM2G134866_P02 DUF573
GRMZM2G147515_P01 DUF573
GRMZM2G162405_P01 DUF573
GRMZM2G162405_P02 DUF573

General references

Chevalier, F; Perazza, D; Laporte, F; Le Hénanff, G; Hornitschek, P; Bonneville, JM; Herzog, M; Vachon, G. 2008. GeBP and GeBP-like proteins are noncanonical leucine-zipper transcription factors that regulate cytokinin response in Arabidopsis. Plant Physiol. 146(3):1142-54 PUBMEDID:18162594
Curaba, J; Herzog, M; Vachon, G. 2003. GeBP, the first member of a new gene family in Arabidopsis, encodes a nuclear protein with DNA-binding activity and is regulated by KNAT1. Plant J. 33(2):305-17 PUBMEDID:12535344