Arabidopsis lyrata MED6 Family
Description
Lee et al. 1997: A temperature-sensitive mutation was obtained in Med6p, a component of the mediator complex from the yeast Saccharomyces cerevisiae. The mediator complex has been shown to enable transcriptional activation in vitro. This mutation in Med6p abolished activation of transcription from four of five inducible promoters tested in vivo. There was no effect, however, on uninduced transcription, transcription of constitutively expressed genes, or transcription by RNA polymerases I and III. Mediator-RNA polymerase II complex isolated from the mutant yeast strain was temperature sensitive for transcriptional activation in a reconstituted in vitro system due to a defect in initiation complex formation. A database search revealed the existence of MED6-related genes in humans and Caenorhabditis elegans, suggesting that the role of mediator in transcriptional activation is conserved throughout the evolution.
Members of this familySHOULD possess MED6 domain
This family is also present in:
- Cyanidioschyzon merolae
- Galdieria sulphuraria
- Chlorella sp. NC64A
- Physcomitrella patens
- Selaginella moellendorffii
- Oryza sativa subsp. indica
- Oryza sativa subsp. japonica
- Sorghum bicolor
- Zea mays
- Arabidopsis thaliana
- Carica papaya
- Populus trichocarpa
- Vitis vinifera
There are 1 gene models in this family
General references
Kim, YJ; Björklund, S; Li, Y; Sayre, MH; Kornberg, RD. 1994. A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II. Cell 77(4):599-608 PUBMEDID:8187178Lee, YC; Min, S; Gim, BS; Kim, YJ. 1997. A transcriptional mediator protein that is required for activation of many RNA polymerase II promoters and is conserved from yeast to humans. Mol. Cell. Biol. 17(8):4622-32 PUBMEDID:9234719
Design qRT-PCR primers using Quantprime