Plant Transcription factor database - Universität Potsdam
version: 3.0

Arabidopsis lyrata PLATZ Family

Description

Nagano et al. 2001: Complementary DNA encoding a DNA-binding protein, designated PLATZ1 (plant AT-rich sequence- and zinc-binding protein 1), was isolated from peas. The amino acid sequence of the protein is similar to those of other uncharacterized proteins predicted from the genome sequences of higher plants. However, no paralogous sequences have been found outside the plant kingdom. Multiple alignments among these paralogous proteins show that several cysteine and histidine residues are invariant, suggesting that these proteins are a novel class of zinc-dependent DNA-binding proteins with two distantly located regions, C-x(2)-H-x(11)-C-x(2)-C-x((4-5))-C-x(2)-C-x((3-7))-H-x(2)-H and C-x(2)-C-x((10-11))-C-x(3)-C. In an electrophoretic mobility shift assay, the zinc chelator 1,10-o-phenanthroline inhibited DNA binding, and two distant zinc-binding regions were required for DNA binding. A protein blot with (65)ZnCl(2) showed that both regions are required for zinc-binding activity. The PLATZ1 protein non-specifically binds to A/T-rich sequences, including the upstream region of the pea GTPase pra2 and plastocyanin petE genes. Expression of the PLATZ1 repressed those of the reporter constructs containing the coding sequence of luciferase gene driven by the cauliflower mosaic virus (CaMV) 35S90 promoter fused to the tandem repeat of the A/T-rich sequences. These results indicate that PLATZ1 is a novel class of plant-specific zinc-dependent DNA-binding protein responsible for A/T-rich sequence-mediated transcriptional repression.

Members of this family
  SHOULD possess PLATZ domain

Domain alignments

This family is also present in:

References

There are 12 gene models in this family



Gene modelDescriptionDomains
313743 fgenesh1_pm.C_scaffold_1002523 PLATZ
476817 fgenesh2_kg.2__1947__AT1G76590.1 PLATZ
480295 fgenesh2_kg.3__2996__AT2G12646.1 PLATZ
484097 fgenesh2_kg.5__89__AT2G01818.1 PLATZ
486540 fgenesh2_kg.5__2532__AT3G60670.1 PLATZ
493082 fgenesh2_kg.7__2515__AT4G17900.1 PLATZ
869030 Al_scaffold_0004_1193 PLATZ
881574 Al_scaffold_0001_3341 PLATZ
882025 Al_scaffold_0001_3792 PLATZ
889557 scaffold_102351.1 PLATZ
899550 scaffold_303520.1 PLATZ
916757 scaffold_800157.1 PLATZ

General references

Nagano, Y; Furuhashi, H; Inaba, T; Sasaki, Y. 2001. A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. Nucleic Acids Res. 29(20):4097-105 PUBMEDID:11600698