Plant Transcription factor database - Universität Potsdam
version: 3.0

Arabidopsis thaliana PLATZ Family

Description

Nagano et al. 2001: Complementary DNA encoding a DNA-binding protein, designated PLATZ1 (plant AT-rich sequence- and zinc-binding protein 1), was isolated from peas. The amino acid sequence of the protein is similar to those of other uncharacterized proteins predicted from the genome sequences of higher plants. However, no paralogous sequences have been found outside the plant kingdom. Multiple alignments among these paralogous proteins show that several cysteine and histidine residues are invariant, suggesting that these proteins are a novel class of zinc-dependent DNA-binding proteins with two distantly located regions, C-x(2)-H-x(11)-C-x(2)-C-x((4-5))-C-x(2)-C-x((3-7))-H-x(2)-H and C-x(2)-C-x((10-11))-C-x(3)-C. In an electrophoretic mobility shift assay, the zinc chelator 1,10-o-phenanthroline inhibited DNA binding, and two distant zinc-binding regions were required for DNA binding. A protein blot with (65)ZnCl(2) showed that both regions are required for zinc-binding activity. The PLATZ1 protein non-specifically binds to A/T-rich sequences, including the upstream region of the pea GTPase pra2 and plastocyanin petE genes. Expression of the PLATZ1 repressed those of the reporter constructs containing the coding sequence of luciferase gene driven by the cauliflower mosaic virus (CaMV) 35S90 promoter fused to the tandem repeat of the A/T-rich sequences. These results indicate that PLATZ1 is a novel class of plant-specific zinc-dependent DNA-binding protein responsible for A/T-rich sequence-mediated transcriptional repression.

Members of this family
  SHOULD possess PLATZ domain

Domain alignments

This family is also present in:

References

There are 14 gene models in this family



Gene modelDescriptionDomains
AT1G21000.1 zinc-binding family protein PLATZ
AT1G21000.2 zinc-binding family protein PLATZ
AT1G31040.1 zinc ion binding PLATZ
AT1G32700.1 zinc-binding family protein PLATZ
AT1G32700.2 zinc-binding family protein PLATZ
AT1G43000.1 zinc-binding family protein PLATZ
AT1G76590.1 zinc-binding family protein PLATZ
AT2G01818.1 zinc ion binding PLATZ
AT2G12646.1 similar to zinc-binding protein, putative [Arabidopsis thaliana] (TAIR:AT3G60670.1); similar to unnamed protein product [Vitis vinifera] (GB:CAO67744.1); contains InterPro domain Protein of unknown function DUF597 (InterPro:IPR006734) PLATZ
AT2G27930.1 zinc-binding family protein PLATZ
AT3G60670.1 zinc-binding protein, putative PLATZ
AT4G17900.1 zinc-binding family protein PLATZ
AT4G17900.2 zinc-binding family protein PLATZ
AT5G46710.1 zinc-binding family protein PLATZ

General references

Nagano, Y; Furuhashi, H; Inaba, T; Sasaki, Y. 2001. A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. Nucleic Acids Res. 29(20):4097-105 PUBMEDID:11600698