Plant Transcription factor database - Universität Potsdam
version: 3.0

Oryza sativa subsp. indica PLATZ Family

Description

Nagano et al. 2001: Complementary DNA encoding a DNA-binding protein, designated PLATZ1 (plant AT-rich sequence- and zinc-binding protein 1), was isolated from peas. The amino acid sequence of the protein is similar to those of other uncharacterized proteins predicted from the genome sequences of higher plants. However, no paralogous sequences have been found outside the plant kingdom. Multiple alignments among these paralogous proteins show that several cysteine and histidine residues are invariant, suggesting that these proteins are a novel class of zinc-dependent DNA-binding proteins with two distantly located regions, C-x(2)-H-x(11)-C-x(2)-C-x((4-5))-C-x(2)-C-x((3-7))-H-x(2)-H and C-x(2)-C-x((10-11))-C-x(3)-C. In an electrophoretic mobility shift assay, the zinc chelator 1,10-o-phenanthroline inhibited DNA binding, and two distant zinc-binding regions were required for DNA binding. A protein blot with (65)ZnCl(2) showed that both regions are required for zinc-binding activity. The PLATZ1 protein non-specifically binds to A/T-rich sequences, including the upstream region of the pea GTPase pra2 and plastocyanin petE genes. Expression of the PLATZ1 repressed those of the reporter constructs containing the coding sequence of luciferase gene driven by the cauliflower mosaic virus (CaMV) 35S90 promoter fused to the tandem repeat of the A/T-rich sequences. These results indicate that PLATZ1 is a novel class of plant-specific zinc-dependent DNA-binding protein responsible for A/T-rich sequence-mediated transcriptional repression.

Members of this family
  SHOULD possess PLATZ domain

Domain alignments

This family is also present in:

References

There are 15 gene models in this family



Gene modelDescriptionDomains
OsIBCD002010 Chr01_3185,711,,,1,711, PLATZ
OsIBCD002012 Chr01_3187,489,,,1,489, PLATZ
OsIBCD005218 Chr02_756,726,,,1,726, PLATZ
OsIBCD005293 Chr02_856,699,,,1,699, PLATZ
OsIBCD005380 Chr02_968,534,,,1,534, PLATZ
OsIBCD007624 Chr02_4641,531,,,1,531, PLATZ
OsIBCD009332 Chr03_1106,810,,,1,810, PLATZ
OsIBCD015416 Chr04_4366,720,,,1,720, PLATZ
OsIBCD021451 Chr06_3921,789,,,1,789, PLATZ
OsIBCD021716 Chr06_4322,843,,,1,843, PLATZ
OsIBCD027414 Chr08_4531,603,,,1,603, PLATZ
OsIBCD027543 Chr09_141,762,,,1,762, PLATZ
OsIBCD027562 Chr09_172,690,,,1,690, PLATZ
OsIBCD031551 Chr10_3395,765,,,1,765, PLATZ
OsIBCD032636 Chr11_1741,243,,,1,243, PLATZ

General references

Nagano, Y; Furuhashi, H; Inaba, T; Sasaki, Y. 2001. A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. Nucleic Acids Res. 29(20):4097-105 PUBMEDID:11600698