Plant Transcription factor database - Universität Potsdam
version: 3.0

Physcomitrella patens PLATZ Family

Description

Nagano et al. 2001: Complementary DNA encoding a DNA-binding protein, designated PLATZ1 (plant AT-rich sequence- and zinc-binding protein 1), was isolated from peas. The amino acid sequence of the protein is similar to those of other uncharacterized proteins predicted from the genome sequences of higher plants. However, no paralogous sequences have been found outside the plant kingdom. Multiple alignments among these paralogous proteins show that several cysteine and histidine residues are invariant, suggesting that these proteins are a novel class of zinc-dependent DNA-binding proteins with two distantly located regions, C-x(2)-H-x(11)-C-x(2)-C-x((4-5))-C-x(2)-C-x((3-7))-H-x(2)-H and C-x(2)-C-x((10-11))-C-x(3)-C. In an electrophoretic mobility shift assay, the zinc chelator 1,10-o-phenanthroline inhibited DNA binding, and two distant zinc-binding regions were required for DNA binding. A protein blot with (65)ZnCl(2) showed that both regions are required for zinc-binding activity. The PLATZ1 protein non-specifically binds to A/T-rich sequences, including the upstream region of the pea GTPase pra2 and plastocyanin petE genes. Expression of the PLATZ1 repressed those of the reporter constructs containing the coding sequence of luciferase gene driven by the cauliflower mosaic virus (CaMV) 35S90 promoter fused to the tandem repeat of the A/T-rich sequences. These results indicate that PLATZ1 is a novel class of plant-specific zinc-dependent DNA-binding protein responsible for A/T-rich sequence-mediated transcriptional repression.

Members of this family
  SHOULD possess PLATZ domain

Domain alignments

This family is also present in:

References

There are 13 gene models in this family



Gene modelDescriptionDomains
111107 e_gw1.1.253.1 PLATZ
112207 e_gw1.3.607.1 PLATZ
112256 e_gw1.3.608.1 PLATZ
113355 e_gw1.6.367.1 PLATZ
121188 e_gw1.35.314.1 PLATZ
173318 estExt_fgenesh1_pg.C_4410003 PLATZ
17927 gw1.66.102.1 PLATZ
17941 gw1.89.80.1 PLATZ
18112 gw1.141.43.1 PLATZ
189465 estExt_gwp_gw1.C_1330066 PLATZ
228346 estExt_Genewise1.C_5600001 PLATZ
7262 gw1.284.25.1 PLATZ
82320 fgenesh1_pg.scaffold_102000043 PLATZ

General references

Nagano, Y; Furuhashi, H; Inaba, T; Sasaki, Y. 2001. A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. Nucleic Acids Res. 29(20):4097-105 PUBMEDID:11600698