Plant Transcription factor database - Universität Potsdam
version: 3.0

Populus trichocarpa PLATZ Family

Description

Nagano et al. 2001: Complementary DNA encoding a DNA-binding protein, designated PLATZ1 (plant AT-rich sequence- and zinc-binding protein 1), was isolated from peas. The amino acid sequence of the protein is similar to those of other uncharacterized proteins predicted from the genome sequences of higher plants. However, no paralogous sequences have been found outside the plant kingdom. Multiple alignments among these paralogous proteins show that several cysteine and histidine residues are invariant, suggesting that these proteins are a novel class of zinc-dependent DNA-binding proteins with two distantly located regions, C-x(2)-H-x(11)-C-x(2)-C-x((4-5))-C-x(2)-C-x((3-7))-H-x(2)-H and C-x(2)-C-x((10-11))-C-x(3)-C. In an electrophoretic mobility shift assay, the zinc chelator 1,10-o-phenanthroline inhibited DNA binding, and two distant zinc-binding regions were required for DNA binding. A protein blot with (65)ZnCl(2) showed that both regions are required for zinc-binding activity. The PLATZ1 protein non-specifically binds to A/T-rich sequences, including the upstream region of the pea GTPase pra2 and plastocyanin petE genes. Expression of the PLATZ1 repressed those of the reporter constructs containing the coding sequence of luciferase gene driven by the cauliflower mosaic virus (CaMV) 35S90 promoter fused to the tandem repeat of the A/T-rich sequences. These results indicate that PLATZ1 is a novel class of plant-specific zinc-dependent DNA-binding protein responsible for A/T-rich sequence-mediated transcriptional repression.

Members of this family
  SHOULD possess PLATZ domain

Domain alignments

This family is also present in:

References

There are 20 gene models in this family



Gene modelDescriptionDomains
242339 gw1.XIII.2588.1 PLATZ
286278 gw1.40.38.1 PLATZ
412468 gw1.II.3803.1 PLATZ
554021 eugene3.00030654 PLATZ
558344 eugene3.00091604 PLATZ
566415 eugene3.00100973 PLATZ
583639 eugene3.01520043 PLATZ
589527 eugene3.00280263 PLATZ
710669 estExt_Genewise1_v1.C_LG_II2106 PLATZ
735546 estExt_Genewise1_v1.C_LG_XVI3058 PLATZ
738385 estExt_Genewise1_v1.C_LG_XIX0919 PLATZ
757009 fgenesh4_pg.C_LG_III000551 PLATZ
760400 fgenesh4_pg.C_LG_V000452 PLATZ
766209 fgenesh4_pg.C_LG_VIII001223 PLATZ
769492 fgenesh4_pg.C_LG_X000982 PLATZ
779642 fgenesh4_pg.C_LG_XVIII001010 PLATZ
813242 fgenesh4_pm.C_scaffold_29000018 PLATZ
815186 estExt_fgenesh4_pg.C_LG_I1220 PLATZ
826609 estExt_fgenesh4_pg.C_290211 PLATZ
831731 estExt_fgenesh4_pm.C_LG_V0730 PLATZ

General references

Nagano, Y; Furuhashi, H; Inaba, T; Sasaki, Y. 2001. A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. Nucleic Acids Res. 29(20):4097-105 PUBMEDID:11600698