Plant Transcription factor database - Universität Potsdam
version: 3.0

Sorghum bicolor PLATZ Family

Description

Nagano et al. 2001: Complementary DNA encoding a DNA-binding protein, designated PLATZ1 (plant AT-rich sequence- and zinc-binding protein 1), was isolated from peas. The amino acid sequence of the protein is similar to those of other uncharacterized proteins predicted from the genome sequences of higher plants. However, no paralogous sequences have been found outside the plant kingdom. Multiple alignments among these paralogous proteins show that several cysteine and histidine residues are invariant, suggesting that these proteins are a novel class of zinc-dependent DNA-binding proteins with two distantly located regions, C-x(2)-H-x(11)-C-x(2)-C-x((4-5))-C-x(2)-C-x((3-7))-H-x(2)-H and C-x(2)-C-x((10-11))-C-x(3)-C. In an electrophoretic mobility shift assay, the zinc chelator 1,10-o-phenanthroline inhibited DNA binding, and two distant zinc-binding regions were required for DNA binding. A protein blot with (65)ZnCl(2) showed that both regions are required for zinc-binding activity. The PLATZ1 protein non-specifically binds to A/T-rich sequences, including the upstream region of the pea GTPase pra2 and plastocyanin petE genes. Expression of the PLATZ1 repressed those of the reporter constructs containing the coding sequence of luciferase gene driven by the cauliflower mosaic virus (CaMV) 35S90 promoter fused to the tandem repeat of the A/T-rich sequences. These results indicate that PLATZ1 is a novel class of plant-specific zinc-dependent DNA-binding protein responsible for A/T-rich sequence-mediated transcriptional repression.

Members of this family
  SHOULD possess PLATZ domain

Domain alignments

This family is also present in:

References

There are 16 gene models in this family



Gene modelDescriptionDomains
127642 fgenesh1_pm.C_chr_4000945 PLATZ
130649 fgenesh1_pm.C_chr_10000354 PLATZ
141738 estExt_fgenesh1_pm.C_chr_10629 PLATZ
148745 estExt_fgenesh1_kg.C_chr_40303 PLATZ
4002319 gw1.4.1620.1 PLATZ
4011982 gw1.8.2667.1 PLATZ
4152484 gw1.7.16874.1 PLATZ
4755537 estExt_Genewise1.C_chr_41062 PLATZ
4762078 estExt_Genewise1.C_chr_67295 PLATZ
4808991 e_gw1.4.1590.1 PLATZ
4966803 fgenesh1_pg.C_chr_1003405 PLATZ
5029807 Sb01g033153 PLATZ
5037728 Sb04g006320 PLATZ
5037729 Sb04g006330 PLATZ
5044676 Sb08g005380 PLATZ
5062069 Sb10g026620 PLATZ

General references

Nagano, Y; Furuhashi, H; Inaba, T; Sasaki, Y. 2001. A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. Nucleic Acids Res. 29(20):4097-105 PUBMEDID:11600698