Plant Transcription factor database - Universität Potsdam
version: 3.0

Zea mays PLATZ Family

Description

Nagano et al. 2001: Complementary DNA encoding a DNA-binding protein, designated PLATZ1 (plant AT-rich sequence- and zinc-binding protein 1), was isolated from peas. The amino acid sequence of the protein is similar to those of other uncharacterized proteins predicted from the genome sequences of higher plants. However, no paralogous sequences have been found outside the plant kingdom. Multiple alignments among these paralogous proteins show that several cysteine and histidine residues are invariant, suggesting that these proteins are a novel class of zinc-dependent DNA-binding proteins with two distantly located regions, C-x(2)-H-x(11)-C-x(2)-C-x((4-5))-C-x(2)-C-x((3-7))-H-x(2)-H and C-x(2)-C-x((10-11))-C-x(3)-C. In an electrophoretic mobility shift assay, the zinc chelator 1,10-o-phenanthroline inhibited DNA binding, and two distant zinc-binding regions were required for DNA binding. A protein blot with (65)ZnCl(2) showed that both regions are required for zinc-binding activity. The PLATZ1 protein non-specifically binds to A/T-rich sequences, including the upstream region of the pea GTPase pra2 and plastocyanin petE genes. Expression of the PLATZ1 repressed those of the reporter constructs containing the coding sequence of luciferase gene driven by the cauliflower mosaic virus (CaMV) 35S90 promoter fused to the tandem repeat of the A/T-rich sequences. These results indicate that PLATZ1 is a novel class of plant-specific zinc-dependent DNA-binding protein responsible for A/T-rich sequence-mediated transcriptional repression.

Members of this family
  SHOULD possess PLATZ domain

Domain alignments

This family is also present in:

References

There are 22 gene models in this family



Gene modelDescriptionDomains
AC184784.3_FGP017 PLATZ
AC203748.3_FGP037 PLATZ
AC205255.3_FGP036 PLATZ
GRMZM2G004548_P01 PLATZ
GRMZM2G006585_P01 PLATZ
GRMZM2G015694_P01 PLATZ
GRMZM2G017882_P01 PLATZ
GRMZM2G043507_P01 PLATZ
GRMZM2G049987_P01 PLATZ
GRMZM2G058645_P01 PLATZ
GRMZM2G058645_P02 PLATZ
GRMZM2G070295_P01 PLATZ
GRMZM2G074752_P01 PLATZ
GRMZM2G077495_P01 PLATZ
GRMZM2G086403_P01 PLATZ
GRMZM2G091044_P01 PLATZ
GRMZM2G094168_P01 PLATZ
GRMZM2G110353_P01 PLATZ
GRMZM2G131280_P01 PLATZ
GRMZM2G149249_P01 PLATZ
GRMZM2G154620_P01 PLATZ
GRMZM2G171934_P01 PLATZ

General references

Nagano, Y; Furuhashi, H; Inaba, T; Sasaki, Y. 2001. A novel class of plant-specific zinc-dependent DNA-binding protein that binds to A/T-rich DNA sequences. Nucleic Acids Res. 29(20):4097-105 PUBMEDID:11600698