Plant Transcription factor database - Universität Potsdam
version: 3.0

Chlorella sp. NC64A RWP-RK Family

Description

Schauser et al. 2005: Genetic studies in Lotus japonicus and pea have identified Nin as a core symbiotic gene required for establishing symbiosis between legumes and nitrogen fixing bacteria collectively called Rhizobium. Sequencing of additional Lotus cDNAs combined with analysis of genome sequences from Arabidopsis and rice reveals that Nin homologues in all three species constitute small gene families. In total, the Arabidopsis and rice genomes encode nine and three NIN-like proteins (NLPs), respectively. We present here a bioinformatics analysis and prediction of NLP evolution. On a genome scale we show that in Arabidopsis, this family has evolved through segmental duplication rather than through tandem amplification. Alignment of all predicted NLP protein sequences shows a composition with six conserved modules. In addition, Lotus and pea NLPs contain segments that might characterize NIN proteins of legumes and be of importance for their function in symbiosis. The most conserved region in NLPs, the RWP-RK domain, has secondary structure predictions consistent with DNA binding properties. This motif is shared by several other small proteins in both Arabidopsis and rice. In rice, the RWP-RK domain sequences have diversified significantly more than in Arabidopsis. Database searches reveal that, apart from its presence in Arabidopsis and rice, the motif is also found in the algae Chlamydomonas and in the slime mold Dictyostelium discoideum. Thus, the origin of this putative DNA binding region seems to predate the fungus-plant divide.

Members of this family
  SHOULD possess RWP-RK domain

Domain alignments

This family is also present in:

References

There are 8 gene models in this family



Gene modelDescriptionDomains
140028 IGS.gm_28_00059 RWP-RK
142237 IGS.gm_4_00378 RWP-RK
143606 IGS.gm_6_00180 RWP-RK
145473 IGS.gm_9_00280 RWP-RK
145474 IGS.gm_9_00281 RWP-RK
27756 e_gw1.27.75.1 RWP-RK
53892 fgenesh3_pg.C_scaffold_18000082 RWP-RK
54703 fgenesh3_pg.C_scaffold_24000087 RWP-RK

General references

Schauser, L; Roussis, A; Stiller, J; Stougaard, J. 1999. A plant regulator controlling development of symbiotic root nodules. Nature 402(6758):191-5 PUBMEDID:10647012