Plant Transcription factor database - Universität Potsdam
version: 3.0

Chlamydomonas reinhardtii RWP-RK Family

Description

Schauser et al. 2005: Genetic studies in Lotus japonicus and pea have identified Nin as a core symbiotic gene required for establishing symbiosis between legumes and nitrogen fixing bacteria collectively called Rhizobium. Sequencing of additional Lotus cDNAs combined with analysis of genome sequences from Arabidopsis and rice reveals that Nin homologues in all three species constitute small gene families. In total, the Arabidopsis and rice genomes encode nine and three NIN-like proteins (NLPs), respectively. We present here a bioinformatics analysis and prediction of NLP evolution. On a genome scale we show that in Arabidopsis, this family has evolved through segmental duplication rather than through tandem amplification. Alignment of all predicted NLP protein sequences shows a composition with six conserved modules. In addition, Lotus and pea NLPs contain segments that might characterize NIN proteins of legumes and be of importance for their function in symbiosis. The most conserved region in NLPs, the RWP-RK domain, has secondary structure predictions consistent with DNA binding properties. This motif is shared by several other small proteins in both Arabidopsis and rice. In rice, the RWP-RK domain sequences have diversified significantly more than in Arabidopsis. Database searches reveal that, apart from its presence in Arabidopsis and rice, the motif is also found in the algae Chlamydomonas and in the slime mold Dictyostelium discoideum. Thus, the origin of this putative DNA binding region seems to predate the fungus-plant divide.

Members of this family
  SHOULD possess RWP-RK domain

Domain alignments

This family is also present in:

References

There are 13 gene models in this family



Gene modelDescriptionDomains
174777 fgenesh2_pg.C_scaffold_26000159 RWP-RK
177573 fgenesh2_pg.C_scaffold_43000027 RWP-RK
186995 estExt_fgenesh2_pg.C_30017 RWP-RK
195838 FER_fgenesh1_est.C_scaffold_27000019 RWP-RK
195839 FER_157695 RWP-RK
195840 FER_158808 RWP-RK
195860 FER_estExt_fgenesh2_pg.C_260253 RWP-RK
195865 FER_Chlre2_kg.scaffold_14000013 RWP-RK
195891 FER_158168 RWP-RK
195915 FER_153829 RWP-RK
195916 FER_156992 RWP-RK
195917 FER_171222 RWP-RK
195918 FER_rwp13 RWP-RK

General references

Schauser, L; Roussis, A; Stiller, J; Stougaard, J. 1999. A plant regulator controlling development of symbiotic root nodules. Nature 402(6758):191-5 PUBMEDID:10647012