Plant Transcription factor database - Universität Potsdam
version: 3.0

Physcomitrella patens RWP-RK Family

Description

Schauser et al. 2005: Genetic studies in Lotus japonicus and pea have identified Nin as a core symbiotic gene required for establishing symbiosis between legumes and nitrogen fixing bacteria collectively called Rhizobium. Sequencing of additional Lotus cDNAs combined with analysis of genome sequences from Arabidopsis and rice reveals that Nin homologues in all three species constitute small gene families. In total, the Arabidopsis and rice genomes encode nine and three NIN-like proteins (NLPs), respectively. We present here a bioinformatics analysis and prediction of NLP evolution. On a genome scale we show that in Arabidopsis, this family has evolved through segmental duplication rather than through tandem amplification. Alignment of all predicted NLP protein sequences shows a composition with six conserved modules. In addition, Lotus and pea NLPs contain segments that might characterize NIN proteins of legumes and be of importance for their function in symbiosis. The most conserved region in NLPs, the RWP-RK domain, has secondary structure predictions consistent with DNA binding properties. This motif is shared by several other small proteins in both Arabidopsis and rice. In rice, the RWP-RK domain sequences have diversified significantly more than in Arabidopsis. Database searches reveal that, apart from its presence in Arabidopsis and rice, the motif is also found in the algae Chlamydomonas and in the slime mold Dictyostelium discoideum. Thus, the origin of this putative DNA binding region seems to predate the fungus-plant divide.

Members of this family
  SHOULD possess RWP-RK domain

Domain alignments

This family is also present in:

References

There are 8 gene models in this family



Gene modelDescriptionDomains
102827 fgenesh1_pg.scaffold_823000001 RWP-RK
10989 gw1.109.16.1 PB1 RWP-RK
159049 estExt_fgenesh1_pg.C_90069 RWP-RK
189078 estExt_gwp_gw1.C_1280044 PB1 RWP-RK
205328 estExt_Genewise1.C_260189 PB1 RWP-RK
223145 estExt_Genewise1.C_2500013 PB1 RWP-RK
93906 fgenesh1_pg.scaffold_250000022 RWP-RK
99342 fgenesh1_pg.scaffold_387000004 RWP-RK

General references

Schauser, L; Roussis, A; Stiller, J; Stougaard, J. 1999. A plant regulator controlling development of symbiotic root nodules. Nature 402(6758):191-5 PUBMEDID:10647012