Plant Transcription factor database - Universität Potsdam
version: 3.0

Arabidopsis lyrata SWI/SNF-BAF60b Family

Description

Kussie et al. 1996:The MDM2 oncoprotein is a cellular inhibitor of the p53 tumor suppressor in that it can bind the transactivation domain of p53 and downregulate its ability to activate transcription. In certain cancers, MDM2 amplification is a common event and contributes to the inactivation of p53. The crystal structure of the 109-residue amino-terminal domain of MDM2 bound to a 15-residue transactivation domain peptide of p53 revealed that MDM2 has a deep hydrophobic cleft on which the p53 peptide binds as an amphipathic alpha helix. The interface relies on the steric complementarity between the MDM2 cleft and the hydrophobic face of the p53 alpha helix and, in particular, on a triad of p53 amino acids-Phe19, Trp23, and Leu26-which insert deep into the MDM2 cleft. These same p53 residues are also involved in transactivation, supporting the hypothesis that MDM2 inactivates p53 by concealing its transactivation domain. The structure also suggests that the amphipathic alpha helix may be a common structural motif in the binding of a diverse family of transactivation factors to the TATA-binding protein-associated factors.

Members of this family
  SHOULD possess SWIB domain

Domain alignments

This family is also present in:

References

There are 18 gene models in this family



Gene modelDescriptionDomains
318665 fgenesh1_pm.C_scaffold_3001681 DEK_C SWIB
319846 fgenesh1_pm.C_scaffold_3002862 Plus-3 SWIB
332593 fgenesh1_pm.C_scaffold_8001936 Plus-3 SWIB
351368 fgenesh1_pg.C_scaffold_6001961 Plus-3 SWIB
354113 fgenesh1_pg.C_scaffold_7001286 Hin1 SWIB
473362 fgenesh2_kg.1__3316__AT1G31760.1 SWIB
474089 fgenesh2_kg.1__4043__AT1G49520.1 DEK_C SWIB
477604 fgenesh2_kg.3__305__AT3G03590.1 SWIB
482518 fgenesh2_kg.4__1578__AT2G35605.1 SWIB
487680 fgenesh2_kg.6__814__AT5G08430.1 GYF Plus-3 SWIB
488249 fgenesh2_kg.6__1383__AT5G14170.1 SWIB
491238 fgenesh2_kg.7__671__AT4G34290.1 SWIB
492627 fgenesh2_kg.7__2060__AT4G22360.1 DEK_C SWIB
866198 Al_scaffold_0005_1677 SWIB
896054 scaffold_300024.1 SWIB
899713 scaffold_303683.1 SWIB
900177 scaffold_304147.1 GYF SWIB
910992 scaffold_603049.1 SWIB

General references

Bennett-Lovsey, R; Hart, SE; Shirai, H; Mizuguchi, K. 2002. The SWIB and the MDM2 domains are homologous and share a common fold. Bioinformatics 18(4):626-30 PUBMEDID:12016060
Kussie, PH; Gorina, S; Marechal, V; Elenbaas, B; Moreau, J; Levine, AJ; Pavletich, NP. 1996. Structure of the MDM2 oncoprotein bound to the p53 tumor suppressor transactivation domain. Science 274(5289):948-53 PUBMEDID:8875929