Plant Transcription factor database - Universität Potsdam
version: 3.0

Physcomitrella patens SWI/SNF-SWI3 Family


Da et al. 2006: The SWIRM domain is a module found in the Swi3 and Rsc8 subunits of SWI/SNF-family chromatin remodeling complexes, and the Ada2 and BHC110/LSD1 subunits of chromatin modification complexes. Here we report the high-resolution crystal structure of the SWIRM domain from Swi3 and characterize the in vitro and in vivo function of the SWIRM domains from Saccharomyces cerevisiae Swi3 and Rsc8. The Swi3 SWIRM forms a four-helix bundle containing a pseudo 2-fold axis and a helix-turn-helix motif commonly found in DNA-binding proteins. We show that the Swi3 SWIRM binds free DNA and mononucleosomes with high and comparable affinity and that a subset of Swi3 substitution mutants that display growth defects in vivo also show impaired DNA-binding activity in vitro, consistent with a nucleosome targeting function of this domain. Genetic and biochemical studies also reveal that the Rsc8 and Swi3 SWIRM domains are essential for the proper assembly and in vivo functions of their respective complexes. Together, these studies identify the SWIRM domain as an essential multifunctional module for the regulation of gene expression.

Members of this family
  SHOULD possess SWIRM domain
  SHOULD NOT possess Myb_DNA-binding domain

Domain alignments

This family is also present in:


There are 5 gene models in this family

Gene modelDescriptionDomains
164777 estExt_fgenesh1_pg.C_760080 SWIRM
166589 estExt_fgenesh1_pg.C_1190058 SWIRM
169206 estExt_fgenesh1_pg.C_2000051 Amino_oxidase DAO SWIRM
208738 estExt_Genewise1.C_480072 Amino_oxidase DAO SWIRM
217676 estExt_Genewise1.C_1450051 Amino_oxidase DAO SWIRM

General references

Da, G; Lenkart, J; Zhao, K; Shiekhattar, R; Cairns, BR; Marmorstein, R. 2006. Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes. Proc. Natl. Acad. Sci. U.S.A. 103(7):2057-62 PUBMEDID:16461455