Plant Transcription factor database - Universität Potsdam
version: 3.0

Arabidopsis thaliana TAZ Family

Description

Du &: Poovaiah. 2004: A novel CaM-binding protein was isolated through protein-protein interaction based screening of an Arabidopsis cDNA expression library using a 35S calmodulin (CaM) probe. There are four additional homologs in the Arabidopsis genome with similar structures: a BTB domain in the N-terminus and a Zf-TAZ domain in the C-terminus. Hence, they were designated as AtBT1-5 (Arabidopsis thaliana BTB and TAZ domain protein). CaM-binding experiments revealed that all five AtBTs are CaM-binding proteins, and their CaM-binding domains were mapped to the C-terminus. AtBT homologs are also present in rice, but are not present in human, animal, yeast or other organisms, suggesting that the BTB and TAZ domain proteins are plant-specific. The AtBT1-smGFP fusion protein expressed in tobacco BY-2 cells showed that AtBT1 targets the nucleus. Yeast two-hybrid screening using an AtBT1 fragment as bait identified two interacting proteins (AtBET10 and AtBET9) belonging to the family of fsh/Ring3 class transcription regulators. The BTB domain of the AtBTs is required for the interaction, and this protein-protein interaction was confirmed by GST pull-down. AtBET10 also interacts with AtBT2 and AtBT4, and exhibited a transcriptional activation function in yeast cells. AtBTs exhibit varying responses to different stress stimuli, but all five genes responded rapidly to H2O2 and salicylic acid (SA) treatments. These results suggest that AtBTs play a role in transcriptional regulation, and signal molecules such as Ca2+, H2O2, and SA affect transcriptional machinery by altering the expression and conformation of AtBTs which interact with transcriptional activators such as AtBET10.

Members of this family
  SHOULD possess zf-TAZ domain
  SHOULD NOT possess PHD domain

Domain alignments

This family is also present in:

References

There are 8 gene models in this family



Gene modelDescriptionDomains
AT1G16710.1 HAC12 (HISTONE ACETYLTRANSFERASE OF THE CBP FAMILY 12); H3/H4 histone acetyltransferase/ transcription cofactor DUF906 ZZ zf-TAZ
AT1G55970.1 ATHPCAT2/HAC4 (HISTONE ACETYLTRANSFERASE OF THE CBP FAMILY 4); histone acetyltransferase DUF906 ZZ zf-TAZ
AT1G67220.1 ATHPCAT1/HAC2 (HISTONE ACETYLTRANSFERASE OF THE CBP FAMILY 2); DNA binding / histone acetyltransferase/ transcription cofactor DUF906 ZZ zf-TAZ
AT1G79000.1 HAC1 (P300/CBP ACETYLTRANSFERASE-RELATED PROTEIN 2 GENE); H3/H4 histone acetyltransferase/ transcription cofactor DUF906 ZZ zf-TAZ
AT3G12980.1 ATHPCAT4/HAC5 (HISTONE ACETYLTRANSFERASE OF THE CBP FAMILY 5); H3/H4 histone acetyltransferase/ histone acetyltransferase DUF906 ZZ zf-TAZ
AT4G37610.1 BT5 (BTB and TAZ domain protein 5); protein binding / transcription regulator BTB zf-TAZ
AT5G67480.1 BT4 (BTB AND TAZ DOMAIN PROTEIN 4); protein binding / transcription regulator BTB zf-TAZ
AT5G67480.2 BT4 (BTB AND TAZ DOMAIN PROTEIN 4); protein binding BTB zf-TAZ

General references

Du, L; Poovaiah, BW. 2004. A novel family of Ca2+/calmodulin-binding proteins involved in transcriptional regulation: interaction with fsh/Ring3 class transcription activators. Plant Mol. Biol. 54(4):549-69 PUBMEDID:15316289