Plant Transcription factor database - Universität Potsdam
version: 3.0

Arabidopsis lyrata TCP Family

Description

Kosugi & Ohashi. 2002: The TCP domain is a plant-specific DNA binding domain found in proteins from a diverse array of species, including the cycloidea (cyc) and teosinte branched1 (tb1) gene products and the PCF1 and PCF2 proteins. To understand the role in transcriptional regulation of proteins with this domain, we have analysed the DNA binding and dimerization specificity of the TCP protein family using rice PCF proteins, and further evaluated potential targets for the TCP protein. The seven PCF members including five newly isolated proteins, were able to be grouped into two classes, I and II, based on sequence similarity in the TCP domain. Random binding site selection experiments and electrophoretic mobility shift assays (EMSAs) revealed the consensus DNA binding sequences of these two classes to be distinct but overlapping; GGNCCCAC for class I and GTGGNCCC for class II. The TB1 protein from maize, which belongs to class II, had the same specificity as the rice class II proteins, suggesting the conservation of binding specificity between TCP domains from different species. The yeast 2-hybrid assay and EMSA revealed that these proteins tend to form a homodimer or a heterodimer between members of the same class. We searched predicted 5' flanking sequences of Arabidopsis genes for the consensus binding sequences and found that the consensus sites are distributed in the genome at a considerably lower frequency. We further analysed eight promoters containing the class I consensus TCP sites. The transcriptional activities of six promoters were decreased by a mutation of the TCP binding site, which is consistent with the observation that the class I TCP site can confer transactivation function on a heterologous promoter. These results suggest that the two classes of TCP protein are distinct in DNA binding specificity and transcriptional regulation.

Members of this family
  SHOULD possess TCP domain

Domain alignments

This family is also present in:

References

There are 25 gene models in this family



Gene modelDescriptionDomains
315900 fgenesh1_pm.C_scaffold_2000933 TCP
338960 fgenesh1_pg.C_scaffold_2001059 TCP
473241 fgenesh2_kg.1__3195__AT1G30210.2 TCP
473685 fgenesh2_kg.1__3639__AT1G35560.1 TCP
474447 fgenesh2_kg.1__4401__AT1G53230.1 TCP
475419 fgenesh2_kg.2__549__AT1G58100.1 TCP
476101 fgenesh2_kg.2__1231__AT1G69690.1 TCP
477445 fgenesh2_kg.3__146__AT3G02150.2 TCP
482683 fgenesh2_kg.4__1743__AT2G37000.1 TCP
483704 fgenesh2_kg.4__2764__AT2G45680.1 TCP
484486 fgenesh2_kg.5__478__AT3G27010.1 TCP
485126 fgenesh2_kg.5__1118__AT3G47620.1 TCP
487639 fgenesh2_kg.6__773__AT5G08070.1 TCP
489189 fgenesh2_kg.6__2323__AT5G23280.1 TCP
493022 fgenesh2_kg.7__2455__AT4G18390.1 TCP
493876 fgenesh2_kg.7__3309__AT5G41030.1 TCP
495258 fgenesh2_kg.8__1074__AT5G51910.2 TCP
496250 fgenesh2_kg.8__2066__AT5G60970.1 TCP
895047 scaffold_202121.1 TCP
897773 scaffold_301743.1 TCP
898223 scaffold_302193.1 TCP
902126 scaffold_401627.1 TCP
905655 scaffold_501359.1 TCP
905662 scaffold_501366.1 TCP
908782 scaffold_600839.1 TCP

General references

Cubas, P; Lauter, N; Doebley, J; Coen, E. 1999. The TCP domain: a motif found in proteins regulating plant growth and development. Plant J. 18(2):215-22 PUBMEDID:10363373
Kosugi, S; Ohashi, Y. 2002. DNA binding and dimerization specificity and potential targets for the TCP protein family. Plant J. 30(3):337-48 PUBMEDID:12000681
Reeves, PA; Olmstead, RG. 2003. Evolution of the TCP gene family in Asteridae: cladistic and network approaches to understanding regulatory gene family diversification and its impact on morphological evolution. Mol. Biol. Evol. 20(12):1997-2009 PUBMEDID:12885953