Plant Transcription factor database - Universität Potsdam
version: 3.0

Oryza sativa subsp. indica TCP Family


Kosugi & Ohashi. 2002: The TCP domain is a plant-specific DNA binding domain found in proteins from a diverse array of species, including the cycloidea (cyc) and teosinte branched1 (tb1) gene products and the PCF1 and PCF2 proteins. To understand the role in transcriptional regulation of proteins with this domain, we have analysed the DNA binding and dimerization specificity of the TCP protein family using rice PCF proteins, and further evaluated potential targets for the TCP protein. The seven PCF members including five newly isolated proteins, were able to be grouped into two classes, I and II, based on sequence similarity in the TCP domain. Random binding site selection experiments and electrophoretic mobility shift assays (EMSAs) revealed the consensus DNA binding sequences of these two classes to be distinct but overlapping; GGNCCCAC for class I and GTGGNCCC for class II. The TB1 protein from maize, which belongs to class II, had the same specificity as the rice class II proteins, suggesting the conservation of binding specificity between TCP domains from different species. The yeast 2-hybrid assay and EMSA revealed that these proteins tend to form a homodimer or a heterodimer between members of the same class. We searched predicted 5' flanking sequences of Arabidopsis genes for the consensus binding sequences and found that the consensus sites are distributed in the genome at a considerably lower frequency. We further analysed eight promoters containing the class I consensus TCP sites. The transcriptional activities of six promoters were decreased by a mutation of the TCP binding site, which is consistent with the observation that the class I TCP site can confer transactivation function on a heterologous promoter. These results suggest that the two classes of TCP protein are distinct in DNA binding specificity and transcriptional regulation.

Members of this family
  SHOULD possess TCP domain

Domain alignments

This family is also present in:


There are 22 gene models in this family

Gene modelDescriptionDomains
OsIBCD000714 Chr01_1077,1818,,,1,1818, TCP
OsIBCD003342 Chr01_5190,1353,,,1,1353, TCP
OsIBCD007363 Chr02_4229,567,,,1,567, TCP
OsIBCD007969 Chr02_5118,897,,,1,897, TCP
OsIBCD014577 Chr04_3149,3111,,,1,3111, FH2 TCP
OsIBCD018586 Chr05_4080,948,,,1,948, TCP
OsIBCD022295 Chr07_319,630,,,1,630, TCP
OsIBCD028523 Chr09_1942,774,,,1,774, TCP
OsIBCD036868 Chr01_5261,924,,,1,924, TCP
OsIBCD037169 Chr01_6640,957,,,1,957, TCP
OsIBCD038056 Chr02_4182,633,,,1,633, TCP
OsIBCD039363 Chr03_5020,966,,,1,966, TCP
OsIBCD039498 Chr03_5664,993,,,1,993, TCP
OsIBCD039790 Chr04_899,330,,,1,330, TCP
OsIBCD040320 Chr04_3786,621,,,1,621, TCP
OsIBCD041824 Chr06_1201,1164,,,1,1164, TCP
OsIBCD042626 Chr07_432,1338,,,1,1338, TCP
OsIBCD043959 Chr08_3296,801,,,1,801, TCP
OsIBCD044188 Chr08_4362,1122,,,1,1122, TCP
OsIBCD044781 Chr09_2885,1146,,,1,1146, TCP
OsIBCD045612 Chr11_55,426,,,1,426, TCP
OsIBCD046936 Chr12_3177,894,,,1,894, TCP

General references

Cubas, P; Lauter, N; Doebley, J; Coen, E. 1999. The TCP domain: a motif found in proteins regulating plant growth and development. Plant J. 18(2):215-22 PUBMEDID:10363373
Kosugi, S; Ohashi, Y. 2002. DNA binding and dimerization specificity and potential targets for the TCP protein family. Plant J. 30(3):337-48 PUBMEDID:12000681
Reeves, PA; Olmstead, RG. 2003. Evolution of the TCP gene family in Asteridae: cladistic and network approaches to understanding regulatory gene family diversification and its impact on morphological evolution. Mol. Biol. Evol. 20(12):1997-2009 PUBMEDID:12885953