Plant Transcription factor database - Universität Potsdam
version: 3.0

Populus trichocarpa TCP Family

Description

Kosugi & Ohashi. 2002: The TCP domain is a plant-specific DNA binding domain found in proteins from a diverse array of species, including the cycloidea (cyc) and teosinte branched1 (tb1) gene products and the PCF1 and PCF2 proteins. To understand the role in transcriptional regulation of proteins with this domain, we have analysed the DNA binding and dimerization specificity of the TCP protein family using rice PCF proteins, and further evaluated potential targets for the TCP protein. The seven PCF members including five newly isolated proteins, were able to be grouped into two classes, I and II, based on sequence similarity in the TCP domain. Random binding site selection experiments and electrophoretic mobility shift assays (EMSAs) revealed the consensus DNA binding sequences of these two classes to be distinct but overlapping; GGNCCCAC for class I and GTGGNCCC for class II. The TB1 protein from maize, which belongs to class II, had the same specificity as the rice class II proteins, suggesting the conservation of binding specificity between TCP domains from different species. The yeast 2-hybrid assay and EMSA revealed that these proteins tend to form a homodimer or a heterodimer between members of the same class. We searched predicted 5' flanking sequences of Arabidopsis genes for the consensus binding sequences and found that the consensus sites are distributed in the genome at a considerably lower frequency. We further analysed eight promoters containing the class I consensus TCP sites. The transcriptional activities of six promoters were decreased by a mutation of the TCP binding site, which is consistent with the observation that the class I TCP site can confer transactivation function on a heterologous promoter. These results suggest that the two classes of TCP protein are distinct in DNA binding specificity and transcriptional regulation.

Members of this family
  SHOULD possess TCP domain

Domain alignments

This family is also present in:

References

There are 33 gene models in this family



Gene modelDescriptionDomains
197397 gw1.IV.2486.1 TCP
197953 gw1.IV.3042.1 TCP
253757 gw1.XV.3193.1 TCP
266145 gw1.123.167.1 TCP
266526 gw1.124.176.1 TCP
422658 gw1.XII.1118.1 TCP
548532 eugene3.00010973 TCP
549725 eugene3.00012166 TCP
554293 eugene3.00030926 TCP
554743 eugene3.00031376 TCP
556378 eugene3.00041091 TCP
558669 eugene3.00050284 TCP
568415 eugene3.00110429 TCP
568617 eugene3.00110631 TCP
570503 eugene3.00121156 TCP
574356 eugene3.00190830 TCP
589460 eugene3.00280196 TCP
592176 eugene3.00410019 TCP
642377 grail3.0032015302 TCP
650493 grail3.0001135101 TCP
654962 grail3.0011030101 TCP
656764 grail3.0010045201 TCP
658950 grail3.0006038101 TCP
678125 grail3.0041007901 TCP
680833 grail3.0082000401 TCP
750930 fgenesh4_pg.C_LG_I000533 TCP
755220 fgenesh4_pg.C_LG_II001400 TCP
774325 fgenesh4_pg.C_LG_XIV000235 TCP
778619 fgenesh4_pg.C_LG_XVII000498 TCP
780609 fgenesh4_pg.C_LG_XIX000748 TCP
783149 fgenesh4_pg.C_scaffold_86000009 TCP
786035 fgenesh4_pg.C_scaffold_160000018 TCP
827565 estExt_fgenesh4_pg.C_880085 TCP

General references

Cubas, P; Lauter, N; Doebley, J; Coen, E. 1999. The TCP domain: a motif found in proteins regulating plant growth and development. Plant J. 18(2):215-22 PUBMEDID:10363373
Kosugi, S; Ohashi, Y. 2002. DNA binding and dimerization specificity and potential targets for the TCP protein family. Plant J. 30(3):337-48 PUBMEDID:12000681
Reeves, PA; Olmstead, RG. 2003. Evolution of the TCP gene family in Asteridae: cladistic and network approaches to understanding regulatory gene family diversification and its impact on morphological evolution. Mol. Biol. Evol. 20(12):1997-2009 PUBMEDID:12885953