Plant Transcription factor database - Universität Potsdam
version: 3.0

Sorghum bicolor TCP Family

Description

Kosugi & Ohashi. 2002: The TCP domain is a plant-specific DNA binding domain found in proteins from a diverse array of species, including the cycloidea (cyc) and teosinte branched1 (tb1) gene products and the PCF1 and PCF2 proteins. To understand the role in transcriptional regulation of proteins with this domain, we have analysed the DNA binding and dimerization specificity of the TCP protein family using rice PCF proteins, and further evaluated potential targets for the TCP protein. The seven PCF members including five newly isolated proteins, were able to be grouped into two classes, I and II, based on sequence similarity in the TCP domain. Random binding site selection experiments and electrophoretic mobility shift assays (EMSAs) revealed the consensus DNA binding sequences of these two classes to be distinct but overlapping; GGNCCCAC for class I and GTGGNCCC for class II. The TB1 protein from maize, which belongs to class II, had the same specificity as the rice class II proteins, suggesting the conservation of binding specificity between TCP domains from different species. The yeast 2-hybrid assay and EMSA revealed that these proteins tend to form a homodimer or a heterodimer between members of the same class. We searched predicted 5' flanking sequences of Arabidopsis genes for the consensus binding sequences and found that the consensus sites are distributed in the genome at a considerably lower frequency. We further analysed eight promoters containing the class I consensus TCP sites. The transcriptional activities of six promoters were decreased by a mutation of the TCP binding site, which is consistent with the observation that the class I TCP site can confer transactivation function on a heterologous promoter. These results suggest that the two classes of TCP protein are distinct in DNA binding specificity and transcriptional regulation.

Members of this family
  SHOULD possess TCP domain

Domain alignments

This family is also present in:

References

There are 27 gene models in this family



Gene modelDescriptionDomains
129607 fgenesh1_pm.C_chr_8000580 TCP
4144198 gw1.7.15950.1 TCP
4779497 e_gw1.1.2625.1 TCP
4792136 e_gw1.2.17093.1 TCP
4974539 fgenesh1_pg.C_chr_3001654 TCP
4974540 fgenesh1_pg.C_chr_3001655 TCP
4975841 fgenesh1_pg.C_chr_3002956 TCP
4975890 fgenesh1_pg.C_chr_3003005 TCP
4979401 fgenesh1_pg.C_chr_4002234 TCP
4979515 fgenesh1_pg.C_chr_4002348 TCP
4984750 fgenesh1_pg.C_chr_6001637 TCP
4999259 estExt_fgenesh1_pg.C_chr_20329 TCP
5001228 estExt_fgenesh1_pg.C_chr_30191 TCP
5006021 estExt_fgenesh1_pg.C_chr_60252 TCP
5006237 estExt_fgenesh1_pg.C_chr_60886 TCP
5028205 Sb01g006020 TCP
5034162 Sb02g030260 TCP
5035722 Sb03g013360 TCP
5039690 Sb05g004800 TCP
5044612 Sb08g004600 TCP
5044613 Sb08g004610 TCP
5045386 Sb08g019710 TCP
5045547 Sb08g021725 TCP
5054291 Sb03g044320 TCP
5056188 Sb04g038140 TCP
5060787 Sb09g025340 TCP
5061540 Sb10g008030 TCP

General references

Cubas, P; Lauter, N; Doebley, J; Coen, E. 1999. The TCP domain: a motif found in proteins regulating plant growth and development. Plant J. 18(2):215-22 PUBMEDID:10363373
Kosugi, S; Ohashi, Y. 2002. DNA binding and dimerization specificity and potential targets for the TCP protein family. Plant J. 30(3):337-48 PUBMEDID:12000681
Reeves, PA; Olmstead, RG. 2003. Evolution of the TCP gene family in Asteridae: cladistic and network approaches to understanding regulatory gene family diversification and its impact on morphological evolution. Mol. Biol. Evol. 20(12):1997-2009 PUBMEDID:12885953