Plant Transcription factor database - Universität Potsdam
version: 3.0

Zea mays TCP Family

Description

Kosugi & Ohashi. 2002: The TCP domain is a plant-specific DNA binding domain found in proteins from a diverse array of species, including the cycloidea (cyc) and teosinte branched1 (tb1) gene products and the PCF1 and PCF2 proteins. To understand the role in transcriptional regulation of proteins with this domain, we have analysed the DNA binding and dimerization specificity of the TCP protein family using rice PCF proteins, and further evaluated potential targets for the TCP protein. The seven PCF members including five newly isolated proteins, were able to be grouped into two classes, I and II, based on sequence similarity in the TCP domain. Random binding site selection experiments and electrophoretic mobility shift assays (EMSAs) revealed the consensus DNA binding sequences of these two classes to be distinct but overlapping; GGNCCCAC for class I and GTGGNCCC for class II. The TB1 protein from maize, which belongs to class II, had the same specificity as the rice class II proteins, suggesting the conservation of binding specificity between TCP domains from different species. The yeast 2-hybrid assay and EMSA revealed that these proteins tend to form a homodimer or a heterodimer between members of the same class. We searched predicted 5' flanking sequences of Arabidopsis genes for the consensus binding sequences and found that the consensus sites are distributed in the genome at a considerably lower frequency. We further analysed eight promoters containing the class I consensus TCP sites. The transcriptional activities of six promoters were decreased by a mutation of the TCP binding site, which is consistent with the observation that the class I TCP site can confer transactivation function on a heterologous promoter. These results suggest that the two classes of TCP protein are distinct in DNA binding specificity and transcriptional regulation.

Members of this family
  SHOULD possess TCP domain

Domain alignments

This family is also present in:

References

There are 65 gene models in this family



Gene modelDescriptionDomains
AC195983.3_FGP042 TCP
AC199117.2_FGP034 DnaJ TCP
AC200257.4_FGP042 DnaJ TCP
AC213524.3_FGP026 TCP
GRMZM2G002591_P01 TCP
GRMZM2G003944_P01 TCP
GRMZM2G013570_P01 TCP
GRMZM2G013570_P02 TCP
GRMZM2G015037_P01 TCP
GRMZM2G015037_P02 TCP
GRMZM2G017111_P01 DnaJ TCP
GRMZM2G017592_P01 TCP
GRMZM2G020805_P01 TCP
GRMZM2G024639_P01 TCP
GRMZM2G031639_P01 TCP
GRMZM2G031905_P01 TCP
GRMZM2G034638_P01 TCP
GRMZM2G035944_P01 TCP
GRMZM2G035944_P02 TCP
GRMZM2G042977_P01 TCP
GRMZM2G051561_P01 TCP
GRMZM2G055024_P01 TCP
GRMZM2G058841_P01 DnaJ TCP
GRMZM2G059636_P01 TCP
GRMZM2G060319_P01 TCP
GRMZM2G062711_P01 TCP
GRMZM2G064628_P01 TCP
GRMZM2G077755_P01 TCP
GRMZM2G078077_P01 TCP
GRMZM2G088440_P01 DnaJ TCP
GRMZM2G089361_P01 TCP
GRMZM2G089638_P01 TCP
GRMZM2G092214_P01 TCP
GRMZM2G092214_P02 TCP
GRMZM2G093895_P01 TCP
GRMZM2G096610_P01 TCP
GRMZM2G102538_P01 TCP
GRMZM2G102538_P03 TCP
GRMZM2G105287_P01 TCP
GRMZM2G105287_P02 TCP
GRMZM2G107031_P01 TCP
GRMZM2G108954_P01 TCP
GRMZM2G108954_P02 TCP
GRMZM2G110242_P01 TCP
GRMZM2G113888_P01 TCP
GRMZM2G115516_P01 TCP
GRMZM2G116402_P01 TCP
GRMZM2G120151_P01 TCP
GRMZM2G120151_P02 TCP
GRMZM2G124081_P01 TCP
GRMZM2G135461_P01 DnaJ TCP
GRMZM2G142751_P01 TCP
GRMZM2G144999_P01 TCP
GRMZM2G144999_P02 TCP
GRMZM2G145178_P01 TCP
GRMZM2G152879_P01 TCP
GRMZM2G166687_P01 TCP
GRMZM2G166946_P01 TCP
GRMZM2G166946_P02 TCP
GRMZM2G166946_P03 TCP
GRMZM2G170139_P01 TCP
GRMZM2G170216_P01 TCP
GRMZM2G170232_P01 DnaJ TCP
GRMZM2G178603_P01 TCP
GRMZM2G180568_P01 TCP

General references

Cubas, P; Lauter, N; Doebley, J; Coen, E. 1999. The TCP domain: a motif found in proteins regulating plant growth and development. Plant J. 18(2):215-22 PUBMEDID:10363373
Kosugi, S; Ohashi, Y. 2002. DNA binding and dimerization specificity and potential targets for the TCP protein family. Plant J. 30(3):337-48 PUBMEDID:12000681
Reeves, PA; Olmstead, RG. 2003. Evolution of the TCP gene family in Asteridae: cladistic and network approaches to understanding regulatory gene family diversification and its impact on morphological evolution. Mol. Biol. Evol. 20(12):1997-2009 PUBMEDID:12885953