Plant Transcription factor database - Universität Potsdam
version: 3.0

Arabidopsis thaliana WRKY Family

Description

Wu et al. 2005: WRKY transcription factors, originally isolated from plants contain one or two conserved WRKY domains, about 60 amino acid residues with the WRKYGQK sequence followed by a C2H2 or C2HC zinc finger motif. Evidence is accumulating to suggest that the WRKY proteins play significant roles in responses to biotic and abiotic stresses, and in development. In this research, we identified 102 putative WRKY genes from the rice genome and compared them with those from Arabidopsis. The WRKY genes from rice and Arabidopsis were divided into three groups with several subgroups on the basis of phylogenies and the basic structure of the WRKY domains (WDs). The phylogenetic trees generated from the WDs and the genes indicate that the WRKY gene family arose during evolution through duplication and that the dramatic amplification of rice WRKY genes in group III is due to tandem and segmental gene duplication compared with those of Arabidopsis. The result suggests that some of the rice WRKY genes in group III are evolutionarily more active than those in Arabidopsis, and may have specific roles in monocotyledonous plants. Further, it was possible to identify the presence of WRKY-like genes in protists (Giardia lamblia and Dictyostelium discoideum) and green algae Chlamydomonas reinhardtii through database research, demonstrating the ancient origin of the gene family. The results obtained by alignments of the WDs from different species and other analysis imply that domain gain and loss is a divergent force for expansion of the WRKY gene family, and that a rapid amplification of the WRKY genes predate the divergence of monocots and dicots. On the basis of these results, we believe that genes encoding a single WD may have been derived from the C-terminal WD of the genes harboring two WDs. The conserved intron splicing positions in the WDs of higher plants offer clues about WRKY gene evolution, annotation, and classification.

Members of this family
  SHOULD possess WRKY domain

Domain alignments

Benchmark against A. thaliana

The Sensitivity and Positive Predictive Value (PPV) were assessed for this family. The data reported by Ulker & Somssich 2004 for A. thaliana were taken as gold standard.

The gold standard reported 71 members for this family, 71 of which are present in ArabTFDB, giving a PPV of 0.99. One additional member not present in ArabTFDB might be false negative, giving a Sensitivity of 0.99.

This family is also present in:

References

There are 88 gene models in this family



Gene modelDescriptionDomains
AT1G13960.1 WRKY4 (WRKY DNA-binding protein 4); transcription factor WRKY
AT1G13960.2 WRKY4 (WRKY DNA-binding protein 4); transcription factor WRKY
AT1G18860.1 WRKY61 (WRKY DNA-binding protein 61); transcription factor WRKY
AT1G29280.1 WRKY65 (WRKY DNA-binding protein 65); transcription factor WRKY
AT1G29860.1 WRKY71 (WRKY DNA-binding protein 71); transcription factor WRKY
AT1G30650.1 WRKY14 (WRKY DNA-binding protein 14); transcription factor WRKY
AT1G55600.1 WRKY10 (MINISEED3); transcription factor WRKY
AT1G62300.1 WRKY6 (WRKY DNA-binding protein 6); transcription factor WRKY
AT1G64000.1 WRKY56 (WRKY DNA-binding protein 56); transcription factor WRKY
AT1G66550.1 WRKY67 (WRKY DNA-binding protein 67); transcription factor WRKY
AT1G66550.2 WRKY67 (WRKY DNA-binding protein 67) WRKY
AT1G66560.1 WRKY64 (WRKY DNA-binding protein 64); transcription factor WRKY
AT1G66600.1 WRKY63 (WRKY DNA-binding protein 63); transcription factor WRKY
AT1G68150.1 WRKY9 (WRKY DNA-binding protein 9); transcription factor WRKY
AT1G69310.1 WRKY57 (WRKY DNA-binding protein 57); transcription factor WRKY
AT1G69310.2 WRKY57 (WRKY DNA-binding protein 57); transcription factor WRKY
AT1G69810.1 WRKY36 (WRKY DNA-binding protein 36); transcription factor WRKY
AT1G80590.1 WRKY66 (WRKY DNA-binding protein 66); transcription factor WRKY
AT1G80840.1 WRKY40 (WRKY DNA-binding protein 40); transcription factor WRKY
AT2G03340.1 WRKY3 (WRKY DNA-binding protein 3); transcription factor WRKY
AT2G04880.1 ZAP1 (WRKY FAMILY TRANSCRIPTION FACTOR 1); transcription activator/ transcription factor WRKY
AT2G04880.2 ZAP1 (WRKY FAMILY TRANSCRIPTION FACTOR 1); transcription activator/ transcription factor WRKY
AT2G21900.1 WRKY59 (WRKY DNA-binding protein 59); transcription factor WRKY
AT2G23320.1 WRKY15 (WRKY DNA-binding protein 15); transcription factor WRKY
AT2G24570.1 WRKY17 (WRKY DNA-binding protein 17); transcription factor WRKY
AT2G25000.1 WRKY60 (WRKY DNA-BINDING PROTEIN 60); transcription factor WRKY
AT2G30250.1 WRKY25 (WRKY DNA-binding protein 25); transcription factor WRKY
AT2G30590.1 WRKY21 (WRKY DNA-binding protein 21); transcription factor WRKY
AT2G34830.1 WRKY35 (WRKY DNA-BINDING PROTEIN 35); transcription factor WRKY
AT2G37260.1 TTG2 (TRANSPARENT TESTA GLABRA 2); transcription factor WRKY
AT2G37260.2 TTG2 (TRANSPARENT TESTA GLABRA 2) WRKY
AT2G38470.1 WRKY33 (WRKY DNA-binding protein 33); transcription factor WRKY
AT2G40740.1 WRKY55 (WRKY DNA-binding protein 55); transcription factor WRKY
AT2G40740.2 WRKY55 (WRKY DNA-binding protein 55) WRKY
AT2G40750.1 WRKY54 (WRKY DNA-binding protein 54); transcription factor WRKY
AT2G44745.1 WRKY family transcription factor WRKY
AT2G46130.1 WRKY43 (WRKY DNA-binding protein 43); transcription factor WRKY
AT2G46130.2 WRKY43 (WRKY DNA-binding protein 43); transcription factor WRKY
AT2G46400.1 WRKY46 (WRKY DNA-binding protein 46); transcription factor WRKY
AT2G47260.1 WRKY23 (WRKY DNA-binding protein 23); transcription factor WRKY
AT3G01080.1 WRKY58 (WRKY DNA-binding protein 58); transcription factor WRKY
AT3G01970.1 WRKY45 (WRKY DNA-binding protein 45); transcription factor WRKY
AT3G04670.1 WRKY39 (WRKY DNA-binding protein 39); transcription factor WRKY
AT3G04670.2 WRKY39 (WRKY DNA-binding protein 39); transcription factor WRKY
AT3G56400.1 WRKY70 (WRKY DNA-binding protein 70); transcription factor WRKY
AT3G58710.1 WRKY69 (WRKY DNA-binding protein 69); transcription factor WRKY
AT3G58710.2 WRKY69 (WRKY DNA-binding protein 69); transcription factor WRKY
AT3G62340.1 WRKY68 (WRKY DNA-binding protein 68); transcription factor WRKY
AT4G01250.1 WRKY22 (WRKY DNA-binding protein 22); transcription factor WRKY
AT4G01720.1 WRKY47 (WRKY DNA-binding protein 47); transcription factor WRKY
AT4G04450.1 WRKY42 (WRKY DNA-binding protein 42); transcription factor WRKY
AT4G11070.1 WRKY41 (WRKY DNA-binding protein 41); transcription factor WRKY
AT4G11070.2 WRKY41 (WRKY DNA-binding protein 41); transcription factor WRKY
AT4G12020.1 WRKY19 (WRKY DNA-binding protein 19); transcription factor LRR_1 LRR_3 NB-ARC PAH WRKY
AT4G12020.2 WRKY19 (WRKY DNA-binding protein 19) LRR_1 LRR_3 NB-ARC PAH Pkinase Pkinase_Tyr WRKY
AT4G18170.1 WRKY28 (WRKY DNA-binding protein 28); transcription factor WRKY
AT4G22070.1 WRKY31 (WRKY DNA-binding protein 31); transcription factor WRKY
AT4G23550.1 WRKY29 (WRKY DNA-binding protein 29); transcription factor WRKY
AT4G23810.1 WRKY53 (WRKY DNA-binding protein 53); DNA binding / protein binding / transcription activator/ transcription factor WRKY
AT4G24240.1 WRKY7 (WRKY DNA-binding protein 7); transcription factor WRKY
AT4G26440.1 WRKY34 (MICROSPORE-SPECIFIC PROMOTER 3); transcription factor WRKY
AT4G26640.1 WRKY20 (WRKY DNA-binding protein 20); transcription factor WRKY
AT4G26640.2 WRKY20 (WRKY DNA-binding protein 20); transcription factor WRKY
AT4G30935.1 WRKY32 (WRKY DNA-binding protein 32); transcription factor FLYWCH WRKY
AT4G31550.1 WRKY11 (WRKY DNA-binding protein 11); transcription factor WRKY
AT4G31550.2 WRKY11 (WRKY DNA-binding protein 11); transcription factor WRKY
AT4G31800.1 WRKY18 (WRKY DNA-binding protein 18); transcription factor WRKY
AT4G31800.2 WRKY18 (WRKY DNA-binding protein 18); transcription factor WRKY
AT4G39410.1 WRKY13 (WRKY DNA-binding protein 13); transcription factor WRKY
AT5G01900.1 WRKY62 (WRKY DNA-binding protein 62); transcription factor WRKY
AT5G07100.1 WRKY26 (WRKY DNA-binding protein 26); transcription factor WRKY
AT5G07100.2 WRKY26 (WRKY DNA-binding protein 26); transcription factor WRKY
AT5G13080.1 WRKY75 (WRKY DNA-BINDING PROTEIN 75); transcription factor WRKY
AT5G15130.1 WRKY72 (WRKY DNA-binding protein 72); transcription factor WRKY
AT5G22570.1 WRKY38 (WRKY DNA-binding protein 38); transcription factor WRKY
AT5G24110.1 WRKY30 (WRKY DNA-binding protein 30); transcription factor WRKY
AT5G26170.1 WRKY50 (WRKY DNA-binding protein 50); transcription factor WRKY
AT5G28650.1 WRKY74 (WRKY DNA-binding protein 74); transcription factor WRKY
AT5G41570.1 WRKY24 (WRKY DNA-binding protein 24); transcription factor WRKY
AT5G43290.1 WRKY49 (WRKY DNA-binding protein 49); transcription factor WRKY
AT5G45050.1 TTR1 (WRKY domain family protein 16); transcription factor LRR_1 LRR_3 NB-ARC WRKY
AT5G45050.2 TTR1 (WRKY domain family protein 16); transcription factor LRR_1 LRR_3 NB-ARC WRKY
AT5G45260.1 RRS1 (RESISTANT TO RALSTONIA SOLANACEARUM 1); transcription factor LRR_1 LRR_3 NB-ARC WRKY
AT5G46350.1 WRKY8 (WRKY DNA-binding protein 8); transcription factor WRKY
AT5G49520.1 WRKY48 (WRKY DNA-binding protein 48); transcription factor WRKY
AT5G52830.1 WRKY27 (WRKY DNA-binding protein 27); transcription factor WRKY
AT5G56270.1 WRKY2 (WRKY DNA-binding protein 2); transcription factor WRKY
AT5G64810.1 WRKY51 (WRKY DNA-binding protein 51); transcription factor WRKY

General references

Eulgem, T; Rushton, PJ; Robatzek, S; Somssich, IE. 2000. The WRKY superfamily of plant transcription factors. Trends Plant Sci. 5(5):199-206 PUBMEDID:10785665
Ulker, B; Somssich, IE. 2004. WRKY transcription factors: from DNA binding towards biological function. Curr. Opin. Plant Biol. 7(5):491-8 PUBMEDID:15337090
Zhang, Y; Wang, L. 2005. The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants. BMC Evol. Biol. 5(1):1 PUBMEDID:15629062

Specific references

Wu, KL; Guo, ZJ; Wang, HH; Li, J. 2005. The WRKY family of transcription factors in rice and Arabidopsis and their origins. DNA Res. 12(1):9-26 PUBMEDID:16106749