Plant Transcription factor database - Universität Potsdam
version: 3.0

Oryza sativa subsp. japonica WRKY Family

Description

Wu et al. 2005: WRKY transcription factors, originally isolated from plants contain one or two conserved WRKY domains, about 60 amino acid residues with the WRKYGQK sequence followed by a C2H2 or C2HC zinc finger motif. Evidence is accumulating to suggest that the WRKY proteins play significant roles in responses to biotic and abiotic stresses, and in development. In this research, we identified 102 putative WRKY genes from the rice genome and compared them with those from Arabidopsis. The WRKY genes from rice and Arabidopsis were divided into three groups with several subgroups on the basis of phylogenies and the basic structure of the WRKY domains (WDs). The phylogenetic trees generated from the WDs and the genes indicate that the WRKY gene family arose during evolution through duplication and that the dramatic amplification of rice WRKY genes in group III is due to tandem and segmental gene duplication compared with those of Arabidopsis. The result suggests that some of the rice WRKY genes in group III are evolutionarily more active than those in Arabidopsis, and may have specific roles in monocotyledonous plants. Further, it was possible to identify the presence of WRKY-like genes in protists (Giardia lamblia and Dictyostelium discoideum) and green algae Chlamydomonas reinhardtii through database research, demonstrating the ancient origin of the gene family. The results obtained by alignments of the WDs from different species and other analysis imply that domain gain and loss is a divergent force for expansion of the WRKY gene family, and that a rapid amplification of the WRKY genes predate the divergence of monocots and dicots. On the basis of these results, we believe that genes encoding a single WD may have been derived from the C-terminal WD of the genes harboring two WDs. The conserved intron splicing positions in the WDs of higher plants offer clues about WRKY gene evolution, annotation, and classification.

Members of this family
  SHOULD possess WRKY domain

Domain alignments

Benchmark against A. thaliana

The Sensitivity and Positive Predictive Value (PPV) were assessed for this family. The data reported by Ulker & Somssich 2004 for A. thaliana were taken as gold standard.

The gold standard reported 71 members for this family, 71 of which are present in ArabTFDB, giving a PPV of 0.99. One additional member not present in ArabTFDB might be false negative, giving a Sensitivity of 0.99.

This family is also present in:

References

There are 129 gene models in this family



Gene modelDescriptionDomains
LOC_Os01g08710.1 OsWRKY79 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g08710.2 OsWRKY79 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g09080.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os01g09100.1 OsWRKY10 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g14440.1 OsWRKY1v2 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g18584.1 OsWRKY9 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g40260.1 OsWRKY77 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g40430.1 OsWRKY27 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g43550.1 OsWRKY12 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g43550.2 OsWRKY12 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g43650.1 OsWRKY11 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g43650.2 OsWRKY11 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g46800.1 OsWRKY15 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g47560.1 OsWRKY16 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g51690.1 OsWRKY59 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g53040.1 OsWRKY14 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g53260.1 OsWRKY23 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g54600.1 OsWRKY13 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g60490.1 OsWRKY22 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g60520.1 WRKY DNA-binding domain containing protein WRKY
LOC_Os01g60540.1 OsWRKY20 - Superfamily of TFs having WRKY and zinc finger domains WRKY
LOC_Os01g60600.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os01g60640.1 OsWRKY21 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g61080.1 OsWRKY24 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os01g62510.1 WRKY DNA binding domain containing protein WRKY
LOC_Os01g62514.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os01g74140.1 OsWRKY17 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os02g08440.1 OsWRKY71 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os02g08440.2 OsWRKY71 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os02g08440.3 OsWRKY71 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os02g08440.4 OsWRKY71 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os02g16540.1 OsWRKY39v2 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os02g26430.1 OsWRKY42 - Superfamily of TFs having WRKY and zinc finger domains, expressed Plant_zn_clust WRKY
LOC_Os02g43560.1 OsWRKY34 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os02g47060.1 OsWRKY66 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os02g53100.1 OsWRKY32 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os03g20550.1 OsWRKY55 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os03g20550.2 OsWRKY55 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os03g21710.1 WRKY45 transcription factor, putative, expressed WRKY
LOC_Os03g33012.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os03g45450.1 OsWRKY60 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os03g53050.1 WRKY transcription factor, putative, expressed Plant_zn_clust WRKY
LOC_Os03g55080.1 OsWRKY3 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os03g55164.1 OsWRKY4 - Superfamily of TFs having WRKY and zinc finger domains WRKY
LOC_Os03g55164.2 OsWRKY4 - Superfamily of TFs having WRKY and zinc finger domains WRKY
LOC_Os03g58420.1 OsWRKY6 - Superfamily of TFs having WRKY and zinc finger domains, expressed Plant_zn_clust WRKY
LOC_Os03g58420.2 OsWRKY6 - Superfamily of TFs having WRKY and zinc finger domains, expressed Plant_zn_clust WRKY
LOC_Os03g63810.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os04g04300.1 retrotransposon protein, putative, unclassified WRKY
LOC_Os04g21950.1 OsWRKY51 - Superfamily of TFs having WRKY and zinc finger domains, expressed Plant_zn_clust WRKY
LOC_Os04g39570.1 OsWRKY35v2 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os04g39570.2 OsWRKY35v2 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os04g46060.1 OsWRKY36 - Superfamily of TFs having WRKY and zinc finger domains WRKY
LOC_Os04g50920.1 OsWRKY37 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os04g51560.1 OsWRKY68 - Superfamily of TFs having WRKY and zinc finger domains, expressed Plant_zn_clust WRKY
LOC_Os05g03900.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os05g03900.2 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os05g04640.1 OsWRKY5 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os05g09020.1 OsWRKY67 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os05g09020.2 OsWRKY67 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os05g14370.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os05g25700.1 OsWRKY75 - Superfamily of TFs having WRKY and zinc finger domains WRKY
LOC_Os05g25770.1 OsWRKY45 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os05g27730.1 OsWRKY53 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os05g39720.1 OsWRKY70 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os05g40060.1 OsWRKY48 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os05g40070.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os05g40080.1 OsWRKY54 - Superfamily of TFs having WRKY and zinc finger domains WRKY
LOC_Os05g45230.1 OsWRKY58 - Superfamily of TFs having WRKY and zinc finger domains WRKY
LOC_Os05g46020.1 OsWRKY7 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os05g49100.1 OsWRKY49 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os05g49210.1 OsWRKY43 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os05g49620.1 OsWRKY19 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os05g50610.1 OsWRKY8v2 - Superfamily of TFs having WRKY and zinc finger domains WRKY
LOC_Os05g50610.2 OsWRKY8v2 - Superfamily of TFs having WRKY and zinc finger domains WRKY
LOC_Os05g50700.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os06g05380.1 OsWRKY73 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os06g06360.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os06g30860.1 OsWRKY31 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os06g44010.1 OsWRKY28 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os07g02060.1 OsWRKY29 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os07g27670.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os07g39480.1 OsWRKY78 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os07g40570.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os07g48260.1 OsWRKY47 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os08g09800.1 transposon protein, putative, unclassified WRKY hATC zf-BED
LOC_Os08g09810.1 transposon protein, putative, unclassified WRKY hATC zf-BED
LOC_Os08g09840.1 transposon protein, putative, unclassified WRKY hATC zf-BED
LOC_Os08g09900.1 transposon protein, putative, unclassified, expressed WRKY hATC zf-BED
LOC_Os08g13840.1 OsWRKY44 - Superfamily of TFs having WRKY and zinc finger domains, expressed Plant_zn_clust WRKY
LOC_Os08g13840.2 OsWRKY44 - Superfamily of TFs having WRKY and zinc finger domains, expressed Plant_zn_clust WRKY
LOC_Os08g17400.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os08g29660.1 OsWRKY69 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os08g38990.1 OsWRKY30 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os08g38990.2 OsWRKY30 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os08g38990.3 OsWRKY30 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os08g38990.4 OsWRKY30 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os09g09630.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os09g16510.1 OsWRKY74 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os09g25060.1 OsWRKY76 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os09g25070.1 OsWRKY62 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os09g25070.2 OsWRKY62 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os09g30400.1 OsWRKY80 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os09g30400.2 OsWRKY80 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os09g30400.3 OsWRKY80 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os10g18099.1 OsWRKY18 - Superfamily of TFs having WRKY and zinc finger domains WRKY
LOC_Os10g42850.1 OsWRKY2 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os11g02470.1 OsWRKY52 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os11g02480.1 OsWRKY46 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os11g02480.2 OsWRKY46 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os11g02520.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os11g02530.1 OsWRKY40 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os11g02540.1 OsWRKY50 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os11g29870.1 OsWRKY72 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os11g45850.1 OsWRKY81 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os11g45920.1 OsWRKY63 - Superfamily of TFs having WRKY and zinc finger domains WRKY
LOC_Os12g01180.1 OsWRKY57v2 - Superfamily of TFs having WRKY and zinc finger domains, expressed Peptidase_C48 WRKY
LOC_Os12g02400.1 WRKY DNA-binding domain containing protein WRKY
LOC_Os12g02420.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os12g02420.2 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os12g02440.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os12g02440.2 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os12g02440.3 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os12g02440.4 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os12g02450.1 OsWRKY64 - Superfamily of TFs having WRKY and zinc finger domains WRKY
LOC_Os12g02470.1 OsWRKY65 - Superfamily of TFs having WRKY and zinc finger domains, expressed WRKY
LOC_Os12g32250.1 WRKY DNA-binding domain containing protein, expressed WRKY
LOC_Os12g40570.1 WRKY transcription factor, putative, expressed Plant_zn_clust WRKY
LOC_Os12g40570.2 WRKY transcription factor, putative, expressed Plant_zn_clust WRKY

General references

Eulgem, T; Rushton, PJ; Robatzek, S; Somssich, IE. 2000. The WRKY superfamily of plant transcription factors. Trends Plant Sci. 5(5):199-206 PUBMEDID:10785665
Ulker, B; Somssich, IE. 2004. WRKY transcription factors: from DNA binding towards biological function. Curr. Opin. Plant Biol. 7(5):491-8 PUBMEDID:15337090
Zhang, Y; Wang, L. 2005. The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants. BMC Evol. Biol. 5(1):1 PUBMEDID:15629062

Specific references

Sun, C; Palmqvist, S; Olsson, H; Borén, M; Ahlandsberg, S; Jansson, C. 2003. A novel WRKY transcription factor, SUSIBA2, participates in sugar signaling in barley by binding to the sugar-responsive elements of the iso1 promoter. Plant Cell 15(9):2076-92 PUBMEDID:12953112
Wu, KL; Guo, ZJ; Wang, HH; Li, J. 2005. The WRKY family of transcription factors in rice and Arabidopsis and their origins. DNA Res. 12(1):9-26 PUBMEDID:16106749