Plant Transcription factor database - Universität Potsdam
version: 3.0

Vitis vinifera WRKY Family


Wu et al. 2005: WRKY transcription factors, originally isolated from plants contain one or two conserved WRKY domains, about 60 amino acid residues with the WRKYGQK sequence followed by a C2H2 or C2HC zinc finger motif. Evidence is accumulating to suggest that the WRKY proteins play significant roles in responses to biotic and abiotic stresses, and in development. In this research, we identified 102 putative WRKY genes from the rice genome and compared them with those from Arabidopsis. The WRKY genes from rice and Arabidopsis were divided into three groups with several subgroups on the basis of phylogenies and the basic structure of the WRKY domains (WDs). The phylogenetic trees generated from the WDs and the genes indicate that the WRKY gene family arose during evolution through duplication and that the dramatic amplification of rice WRKY genes in group III is due to tandem and segmental gene duplication compared with those of Arabidopsis. The result suggests that some of the rice WRKY genes in group III are evolutionarily more active than those in Arabidopsis, and may have specific roles in monocotyledonous plants. Further, it was possible to identify the presence of WRKY-like genes in protists (Giardia lamblia and Dictyostelium discoideum) and green algae Chlamydomonas reinhardtii through database research, demonstrating the ancient origin of the gene family. The results obtained by alignments of the WDs from different species and other analysis imply that domain gain and loss is a divergent force for expansion of the WRKY gene family, and that a rapid amplification of the WRKY genes predate the divergence of monocots and dicots. On the basis of these results, we believe that genes encoding a single WD may have been derived from the C-terminal WD of the genes harboring two WDs. The conserved intron splicing positions in the WDs of higher plants offer clues about WRKY gene evolution, annotation, and classification.

Members of this family
  SHOULD possess WRKY domain

Domain alignments

Benchmark against A. thaliana

The Sensitivity and Positive Predictive Value (PPV) were assessed for this family. The data reported by Ulker & Somssich 2004 for A. thaliana were taken as gold standard.

The gold standard reported 71 members for this family, 71 of which are present in ArabTFDB, giving a PPV of 0.99. One additional member not present in ArabTFDB might be false negative, giving a Sensitivity of 0.99.

This family is also present in:


There are 59 gene models in this family

Gene modelDescriptionDomains
GSVIVP00000107001 WRKY
GSVIVP00002171001 Plant_zn_clust WRKY
GSVIVP00002446001 WRKY
GSVIVP00002535001 WRKY
GSVIVP00002773001 WRKY
GSVIVP00003337001 WRKY
GSVIVP00003403001 WRKY
GSVIVP00004133001 WRKY
GSVIVP00006494001 WRKY
GSVIVP00006872001 WRKY
GSVIVP00007309001 Plant_zn_clust WRKY
GSVIVP00012503001 WRKY
GSVIVP00014872001 Plant_zn_clust WRKY
GSVIVP00017225001 WRKY
GSVIVP00017951001 WRKY
GSVIVP00018784001 WRKY
GSVIVP00018895001 WRKY
GSVIVP00019057001 WRKY
GSVIVP00019365001 WRKY
GSVIVP00019380001 WRKY
GSVIVP00019549001 Plant_zn_clust WRKY
GSVIVP00020895001 WRKY
GSVIVP00021021001 WRKY
GSVIVP00021337001 WRKY
GSVIVP00023080001 WRKY
GSVIVP00023389001 Plant_zn_clust WRKY
GSVIVP00023394001 WRKY
GSVIVP00023396001 WRKY
GSVIVP00023865001 Plant_zn_clust WRKY
GSVIVP00023994001 WRKY
GSVIVP00024838001 WRKY
GSVIVP00025092001 WRKY
GSVIVP00026902001 WRKY
GSVIVP00026906001 WRKY
GSVIVP00027001001 WRKY
GSVIVP00027206001 WRKY
GSVIVP00027287001 WRKY
GSVIVP00028119001 WRKY
GSVIVP00028138001 WRKY
GSVIVP00028232001 WRKY
GSVIVP00029005001 WRKY
GSVIVP00029143001 WRKY
GSVIVP00029465001 WRKY
GSVIVP00029466001 WRKY
GSVIVP00029867001 WRKY
GSVIVP00030063001 WRKY
GSVIVP00031317001 Plant_zn_clust WRKY
GSVIVP00031504001 WRKY
GSVIVP00032230001 WRKY
GSVIVP00032687001 WRKY
GSVIVP00032689001 WRKY
GSVIVP00032777001 WRKY
GSVIVP00033165001 WRKY
GSVIVP00035835001 WRKY
GSVIVP00037418001 WRKY
GSVIVP00037501001 WRKY
GSVIVP00037648001 WRKY
GSVIVP00037762001 WRKY
GSVIVP00037881001 WRKY

General references

Eulgem, T; Rushton, PJ; Robatzek, S; Somssich, IE. 2000. The WRKY superfamily of plant transcription factors. Trends Plant Sci. 5(5):199-206 PUBMEDID:10785665
Ulker, B; Somssich, IE. 2004. WRKY transcription factors: from DNA binding towards biological function. Curr. Opin. Plant Biol. 7(5):491-8 PUBMEDID:15337090
Zhang, Y; Wang, L. 2005. The WRKY transcription factor superfamily: its origin in eukaryotes and expansion in plants. BMC Evol. Biol. 5(1):1 PUBMEDID:15629062