Plant Transcription factor database - Universität Potsdam
version: 3.0

Arabidopsis thaliana zf-HD Family

Description

Windhovel et al. 2001: We are interested in the regulatory mechanisms responsible for the mesophyll-specific expression of C4 phosphoenolpyruvate carboxylase (PEPCase). A one-hybrid screen resulted in the cloning of four different members of a novel class of plant homeodomain proteins, which are most likely involved in the mesophyll-specific expression of the C4 PEPCase gene in C4 species of the genus Flaveria. Inspection of the homeodomains of the four proteins reveals that they share many common features with homeodomains described so far, but there are also significant differences. Interestingly, this class of homeodomain proteins occurs also in Arabidopsis thaliana and other C3 plants. One-hybrid experiments as well as in vitro DNA binding studies confirmed that these novel homeodomain proteins specifically interact with the proximal region of the C4 PEPCase gene. The N-terminal domains of the homeodomain proteins contain highly conserved sequence motifs. Two-hybrid experiments show that these motifs are sufficient to confer homo- or heterodimer formation between the proteins. Mutagenesis of conserved cysteine residues within the dimerization domain indicates that these residues are essential for dimer formation. Therefore, we designate this novel class of homeobox proteins ZF-HD, for zinc finger homeodomain protein. Our data suggest that the ZF-HD class of homeodomain proteins may be involved in the establishment of the characteristic expression pattern of the C4 PEPCase gene.

Members of this family
  SHOULD possess ZF-HD_dimer domain

Domain alignments

This family is also present in:

References

There are 18 gene models in this family



Gene modelDescriptionDomains
AT1G14440.1 ATHB31 (ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 31); transcription factor ZF-HD_dimer
AT1G14440.2 ATHB31 (ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 31); transcription factor ZF-HD_dimer
AT1G14687.1 ATHB32 (ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 32); DNA binding / transcription factor ZF-HD_dimer
AT1G18835.1 MIF3 (MINI ZINC FINGER) ZF-HD_dimer
AT1G69600.1 ATHB29/ZFHD1 (ZINC FINGER HOMEODOMAIN 1); DNA binding / transcription activator/ transcription factor ZF-HD_dimer
AT1G74660.1 MIF1 (MINI ZINC FINGER 1); DNA binding / transcription factor ZF-HD_dimer
AT1G75240.1 ATHB33 (ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 33); DNA binding / transcription factor ZF-HD_dimer
AT2G02540.1 ATHB21/ZFHD4 (ZINC FINGER HOMEODOMAIN 4); DNA binding / transcription factor ZF-HD_dimer
AT2G18350.1 ATHB24 (ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 24); DNA binding / transcription factor ZF-HD_dimer
AT3G28917.1 MIF2 (MINI ZINC FINGER 2); DNA binding ZF-HD_dimer
AT3G28920.1 ATHB34 (ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 34); DNA binding / transcription factor ZF-HD_dimer
AT3G50890.1 ATHB28 (ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 28); DNA binding / transcription factor ZF-HD_dimer
AT4G24660.1 ATHB22/MEE68 (ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 22); DNA binding / transcription factor ZF-HD_dimer
AT5G15210.1 ATHB30/ZFHD3 (ZINC FINGER HOMEODOMAIN 3); DNA binding / transcription factor ZF-HD_dimer
AT5G39760.1 ATHB23 (ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 23); DNA binding / transcription factor ZF-HD_dimer
AT5G42780.1 ATHB27 (ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 27); DNA binding / transcription factor ZF-HD_dimer
AT5G60480.1 ATHB26 (ARABIDOPSIS THALIANA HOMEOBOX PROTEIN 26); DNA binding / transcription factor ZF-HD_dimer
AT5G65410.1 ATHB25/ZFHD2 (ZINC FINGER HOMEODOMAIN 2); transcription factor ZF-HD_dimer

General references

Windhövel, A; Hein, I; Dabrowa, R; Stockhaus, J. 2001. Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia. Plant Mol. Biol. 45(2):201-14 PUBMEDID:11289511

Specific references

Tan, QK; Irish, VF. 2006. The Arabidopsis zinc finger-homeodomain genes encode proteins with unique biochemical properties that are coordinately expressed during floral development. Plant Physiol. 140(3):1095-108 PUBMEDID:16428600