Plant Transcription factor database - Universität Potsdam
version: 3.0

Oryza sativa subsp. indica zf-HD Family


Windhovel et al. 2001: We are interested in the regulatory mechanisms responsible for the mesophyll-specific expression of C4 phosphoenolpyruvate carboxylase (PEPCase). A one-hybrid screen resulted in the cloning of four different members of a novel class of plant homeodomain proteins, which are most likely involved in the mesophyll-specific expression of the C4 PEPCase gene in C4 species of the genus Flaveria. Inspection of the homeodomains of the four proteins reveals that they share many common features with homeodomains described so far, but there are also significant differences. Interestingly, this class of homeodomain proteins occurs also in Arabidopsis thaliana and other C3 plants. One-hybrid experiments as well as in vitro DNA binding studies confirmed that these novel homeodomain proteins specifically interact with the proximal region of the C4 PEPCase gene. The N-terminal domains of the homeodomain proteins contain highly conserved sequence motifs. Two-hybrid experiments show that these motifs are sufficient to confer homo- or heterodimer formation between the proteins. Mutagenesis of conserved cysteine residues within the dimerization domain indicates that these residues are essential for dimer formation. Therefore, we designate this novel class of homeobox proteins ZF-HD, for zinc finger homeodomain protein. Our data suggest that the ZF-HD class of homeodomain proteins may be involved in the establishment of the characteristic expression pattern of the C4 PEPCase gene.

Members of this family
  SHOULD possess ZF-HD_dimer domain

Domain alignments

This family is also present in:


There are 14 gene models in this family

Gene modelDescriptionDomains
OsIBCD007706 Chr02_4758,690,,,1,690, ZF-HD_dimer
OsIBCD014389 Chr04_2885,642,,,1,642, ZF-HD_dimer
OsIBCD014392 Chr04_2888,525,,,1,525, ZF-HD_dimer
OsIBCD028544 Chr09_1970,639,,,1,639, ZF-HD_dimer
OsIBCD032292 Chr11_1103,1074,,,1,1074, ZF-HD_dimer
OsIBCD039390 Chr03_5133,717,,,1,717, ZF-HD_dimer
OsIBCD041511 Chr05_4659,774,,,1,774, ZF-HD_dimer
OsIBCD043966 Chr08_3346,384,,,1,384, ZF-HD_dimer
OsIBCD043968 Chr08_3350,1089,,,1,1089, ZF-HD_dimer
OsIBCD044059 Chr08_3737,873,,,1,873, ZF-HD_dimer
OsIBCD044582 Chr09_1969,342,,,1,342, ZF-HD_dimer
OsIBCD044686 Chr09_2433,840,,,1,840, ZF-HD_dimer
OsIBCD045640 Chr11_189,318,,,1,318, ZF-HD_dimer
OsIBCD046379 Chr12_256,318,,,1,318, ZF-HD_dimer

General references

Windhövel, A; Hein, I; Dabrowa, R; Stockhaus, J. 2001. Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia. Plant Mol. Biol. 45(2):201-14 PUBMEDID:11289511