Plant Transcription factor database - Universität Potsdam
version: 3.0

Oryza sativa subsp. japonica zf-HD Family

Description

Windhovel et al. 2001: We are interested in the regulatory mechanisms responsible for the mesophyll-specific expression of C4 phosphoenolpyruvate carboxylase (PEPCase). A one-hybrid screen resulted in the cloning of four different members of a novel class of plant homeodomain proteins, which are most likely involved in the mesophyll-specific expression of the C4 PEPCase gene in C4 species of the genus Flaveria. Inspection of the homeodomains of the four proteins reveals that they share many common features with homeodomains described so far, but there are also significant differences. Interestingly, this class of homeodomain proteins occurs also in Arabidopsis thaliana and other C3 plants. One-hybrid experiments as well as in vitro DNA binding studies confirmed that these novel homeodomain proteins specifically interact with the proximal region of the C4 PEPCase gene. The N-terminal domains of the homeodomain proteins contain highly conserved sequence motifs. Two-hybrid experiments show that these motifs are sufficient to confer homo- or heterodimer formation between the proteins. Mutagenesis of conserved cysteine residues within the dimerization domain indicates that these residues are essential for dimer formation. Therefore, we designate this novel class of homeobox proteins ZF-HD, for zinc finger homeodomain protein. Our data suggest that the ZF-HD class of homeodomain proteins may be involved in the establishment of the characteristic expression pattern of the C4 PEPCase gene.

Members of this family
  SHOULD possess ZF-HD_dimer domain

Domain alignments

This family is also present in:

References

There are 15 gene models in this family



Gene modelDescriptionDomains
LOC_Os01g44430.1 retrotransposon protein, putative, unclassified Hexapep Pericardin_rpt ZF-HD_dimer
LOC_Os02g47770.1 ZF-HD protein dimerisation region containing protein, expressed ZF-HD_dimer
LOC_Os03g50920.1 ZF-HD protein dimerisation region containing protein, expressed ZF-HD_dimer
LOC_Os04g35500.1 ZF-HD protein dimerisation region containing protein, expressed ZF-HD_dimer
LOC_Os05g50310.1 ZF-HD protein dimerisation region containing protein, expressed ZF-HD_dimer
LOC_Os06g23030.1 ZF-HD protein dimerisation region containing protein, expressed ZF-HD_dimer
LOC_Os08g34010.1 ZF-HD protein dimerisation region containing protein, expressed ZF-HD_dimer
LOC_Os08g37400.1 ZF-HD homeobox protein, putative, expressed ZF-HD_dimer
LOC_Os09g24810.1 ZF-HD protein dimerisation region containing protein, expressed ZF-HD_dimer
LOC_Os09g24820.1 ZF-HD protein dimerisation region containing protein, expressed ZF-HD_dimer
LOC_Os09g29130.1 ZF-HD protein dimerisation region containing protein, expressed ZF-HD_dimer
LOC_Os11g03420.1 ZF-HD protein dimerisation region containing protein, expressed ZF-HD_dimer
LOC_Os11g13930.1 ZF-HD protein dimerisation region containing protein, expressed ZF-HD_dimer
LOC_Os12g03110.1 ZF-HD protein dimerisation region containing protein, expressed ZF-HD_dimer
LOC_Os12g10630.1 ZF-HD protein dimerisation region containing protein, expressed ZF-HD_dimer

General references

Windhövel, A; Hein, I; Dabrowa, R; Stockhaus, J. 2001. Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia. Plant Mol. Biol. 45(2):201-14 PUBMEDID:11289511