Plant Transcription factor database - Universität Potsdam
version: 3.0

Sorghum bicolor zf-HD Family


Windhovel et al. 2001: We are interested in the regulatory mechanisms responsible for the mesophyll-specific expression of C4 phosphoenolpyruvate carboxylase (PEPCase). A one-hybrid screen resulted in the cloning of four different members of a novel class of plant homeodomain proteins, which are most likely involved in the mesophyll-specific expression of the C4 PEPCase gene in C4 species of the genus Flaveria. Inspection of the homeodomains of the four proteins reveals that they share many common features with homeodomains described so far, but there are also significant differences. Interestingly, this class of homeodomain proteins occurs also in Arabidopsis thaliana and other C3 plants. One-hybrid experiments as well as in vitro DNA binding studies confirmed that these novel homeodomain proteins specifically interact with the proximal region of the C4 PEPCase gene. The N-terminal domains of the homeodomain proteins contain highly conserved sequence motifs. Two-hybrid experiments show that these motifs are sufficient to confer homo- or heterodimer formation between the proteins. Mutagenesis of conserved cysteine residues within the dimerization domain indicates that these residues are essential for dimer formation. Therefore, we designate this novel class of homeobox proteins ZF-HD, for zinc finger homeodomain protein. Our data suggest that the ZF-HD class of homeodomain proteins may be involved in the establishment of the characteristic expression pattern of the C4 PEPCase gene.

Members of this family
  SHOULD possess ZF-HD_dimer domain

Domain alignments

This family is also present in:


There are 13 gene models in this family

Gene modelDescriptionDomains
127540 fgenesh1_pm.C_chr_4000843 Homeobox ZF-HD_dimer
4123360 gw1.6.13876.1 ZF-HD_dimer
4153127 gw1.9.16722.1 ZF-HD_dimer
4892500 estExt_Genewise1Plus.C_chr_81619 ZF-HD_dimer
4971074 fgenesh1_pg.C_chr_2002390 ZF-HD_dimer
4981532 fgenesh1_pg.C_chr_5000892 ZF-HD_dimer
4987581 fgenesh1_pg.C_chr_7001671 ZF-HD_dimer
5028409 Sb01g010020 ZF-HD_dimer
5035000 Sb03g001445 ZF-HD_dimer
5043769 Sb07g021440 ZF-HD_dimer
5044404 Sb08g001830 ZF-HD_dimer
5051178 Sb02g024650 ZF-HD_dimer
5056292 Sb05g001690 ZF-HD_dimer

General references

Windhövel, A; Hein, I; Dabrowa, R; Stockhaus, J. 2001. Characterization of a novel class of plant homeodomain proteins that bind to the C4 phosphoenolpyruvate carboxylase gene of Flaveria trinervia. Plant Mol. Biol. 45(2):201-14 PUBMEDID:11289511